Application
Fig1: Western blot analysis of RAP1A on human RAP1A recombinant protein using anti-RAP1A antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of RAP1A on HEK293 (1) and RAP1A-hIgGFc transfected HEK293 (2) cell lysate using anti-RAP1A antibody at 1/1,000 dilution.Application
Fig3: Flow cytometric analysis of HeLa cells with RAP1A antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | RAP1A [B4-G2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C21KG antibody G 22K antibody G-22K antibody GTP binding protein smg p21A antibody GTP-binding protein smg p21A antibody KREV 1 antibody KREV1 antibody OTTHUMP00000013741 antibody RAP 1A antibody RAP1 antibody RAP1A antibody RAP1A member of RAS oncogene family antibody RAP1A_HUMAN antibody Ras related protein Krev 1 antibody Ras related protein Rap 1A antibody RAS related protein RAP1A antibody Ras-related protein Krev-1 antibody Ras-related protein Rap-1A antibody SMGP21 antibody |
| Product description | Two Ras-related human genes, designated RAP1A and RAP1B, encode 95% homologous proteins (namely Rap 1A and Rap 1B) that share a similar C-terminal Cys-Ali-Ali-Xaa sequence with Ras proteins and are ubiquitously expressed in mammalian tissues. The putative "effector" domain of Ras proteins, whose integrity is required for cell transformation as well as interaction with the putative effector protein GAP, is conserved in both Rap 1 proteins. Rap 1A is thought to interfere with Ras effector function by binding to Ras GAP in a GTP-dependent manner without affecting Rap 1A GTPase activity. Rap 2, another Ras-related protein, shares 60% identity with Rap 1A and exhibits a carboxy terminal CAAX motif and two upstream cysteines similar to those of the H-Ras, K-Ras and N-Ras proteins. In contrast with Rap 1A and Rap 1B, overexpression of Rap 2 does not interfere with the Ras signaling pathway. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:1,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | C21KG antibody G 22K antibody G-22K antibody GTP binding protein smg p21A antibody GTP-binding protein smg p21A antibody KREV 1 antibody KREV1 antibody OTTHUMP00000013741 antibody RAP 1A antibody RAP1 antibody RAP1A antibody RAP1A member of RAS oncogene family antibody RAP1A_HUMAN antibody Ras related protein Krev 1 antibody Ras related protein Rap 1A antibody RAS related protein RAP1A antibody Ras-related protein Krev-1 antibody Ras-related protein Rap-1A antibody SMGP21 antibody |
|---|---|
| Molecular Weight(MW) | 21 kDa |
| Cellular Localization | Cell membrane. |
| SwissProt ID | P62834 |
|---|
Application
Fig1: Western blot analysis of RAP1A on human RAP1A recombinant protein using anti-RAP1A antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of RAP1A on HEK293 (1) and RAP1A-hIgGFc transfected HEK293 (2) cell lysate using anti-RAP1A antibody at 1/1,000 dilution.
Application
Fig3: Flow cytometric analysis of HeLa cells with RAP1A antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human RAP1A recombinant protein, RAP1A-hIgGFc transfected HEK293, HeLa. |
|---|---|
| Application Notes | WB:1:500-1:1,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
