Application
Fig1: Western blot analysis of CSNK2A2 on human CSNK2A2 recombinant protein using anti-CSNK2A2 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of CSNK2A2 on different cell lysates using anti-CSNK2A2 antibody at 1/1,000 dilution. Positive control： Lane1: HeLa Lane2: MCF-7 Lane3: HepG2 Lane4: Jurkat Lane5: NIH-3T3 Lane6: PC-12Application
Fig3: Flow cytometric analysis of HepG2 cells with CSNK2A2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | CSNK2A2 [A10-E1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Casein kinase 2 alpha prime polypeptide antibody Casein kinase II alpha' chain antibody Casein kinase II subunit alpha' antibody CK II alpha' antibody CK II antibody CK2A2 antibody CKII antibody CSK22_HUMAN antibody CSNK2A1 antibody CSNK2A2 antibody FLJ43934 antibody |
| Product description | CSNK2A2 also named CKII. Casein kinase I (also designated CKI) and casein kinase II (CKII) compose a family of serine/threonine protein kinases which are present in all eukaryotes examined to date. Casein kinase I family members, which include casein kinase Iα, Iγ, Iδ and Ie, have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. Casein kinase II is usually expressed as a tetrameric complex consisting of either an α2β2 or an αα'β2 structure. The a catalytic subunit is stimulated by the β regulatory subunit, which undergoes autophosphorylation. Casein kinase II activity is high in the cytosol and nucleus of proliferating and differentiating cells. Casein kinase II is known to phosphorylate more than 100 different substrates including nuclear oncoproteins, transcription factors and enzymes involved in DNA metabolism. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:1,000 FC:1:100-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | Casein kinase 2 alpha prime polypeptide antibody Casein kinase II alpha' chain antibody Casein kinase II subunit alpha' antibody CK II alpha' antibody CK II antibody CK2A2 antibody CKII antibody CSK22_HUMAN antibody CSNK2A1 antibody CSNK2A2 antibody FLJ43934 antibody |
|---|---|
| Molecular Weight(MW) | 41 kDa |
| Cellular Localization | Cytosol. Nucleus. |
Application
Fig1: Western blot analysis of CSNK2A2 on human CSNK2A2 recombinant protein using anti-CSNK2A2 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of CSNK2A2 on different cell lysates using anti-CSNK2A2 antibody at 1/1,000 dilution. Positive control： Lane1: HeLa Lane2: MCF-7 Lane3: HepG2 Lane4: Jurkat Lane5: NIH-3T3 Lane6: PC-12
Application
Fig3: Flow cytometric analysis of HepG2 cells with CSNK2A2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human CSNK2A2 recombinant protein, HeLa, MCF-7, HepG2, Jurkat, NIH-3T3, PC-12. |
|---|---|
| Application Notes | WB:1:500-1:1,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
