Application
Fig1: Western blot analysis of ARHGDIA on human ARHGDIA recombinant protein using anti-ARHGDIA antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of ARHGDIA on different cell lysates using anti-ARHGDIA antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: HeLa Lane 3: NIH-3T3 Lane 4: C6 Lane 5: K562 Lane 6: COS7Application
Fig3: ICC staining ARHGDIA (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4:Flow cytometric analysis of Hela cells with ARHGDIA antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | ARHGDIA [G8-C3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ARHGDIA antibody fa96g11 antibody GDIA 1 antibody GDIA1 antibody GDIR1_HUMAN antibody MGC117248 antibody NPHS8 antibody Rho GDI 1 antibody Rho GDI alpha antibody Rho GDI antibody Rho GDP dissociation inhibitor (GDI) alpha antibody Rho GDP dissociation inhibitor 1 antibody Rho GDP dissociation inhibitor alpha antibody Rho GDP-dissociation inhibitor 1 antibody Rho-GDI alpha antibody RhoGDI 1 antibody RhoGDI alpha antibody RHOGDI antibody RhoGDI1 antibody wu:fa96g11 antibody zgc:55554 antibody zgc:77681 antibody |
| Product description | Members of the Ras superfamily of small GTP-binding proteins are critical mediators of diverse cell signaling pathways, including those leading to cell proliferation, cytoskeletal organization and secretion. The counter-conversion of the active GTP-bound form of these proteins to their inactive GDP-bound form is influenced by two types of regulatory proteins: those that alter the intrinsic GTPase activity of the GTP-binding proteins and those that alter the rate of GDP/GTP exchange. Guanine nucleotide-releasing factors (GRFs) increase the GDP dissociation rate, while GDP-dissociation inhibitors (GDIs) decrease the dissociation rate. Rho GDIα, also known as ARHGDIA or GDIA1, is a 204 amino acid member of the Rho GDI family of proteins. Localized to the cytoplasm, Rho GDIα inhibits the dissociation of GDP from Rho proteins, thereby preventing GTP from binding to and subsequently activating Rho proteins. In humans, Rho GDIα can be phosphorylated at Ser 101 by p21-activated kinase (αPAK), an event that inhibits Rho GDIα activity and may result in positive feedback regulation of certain Rho GDIα target proteins. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:500-1:2,000 ICC:1:100-1:500 FC:1:100-1:200 |
|
| Species Reactivity | HumanMouseMonkey |
| Concentration | 2mg/ml |
| Alternative Names | ARHGDIA antibody fa96g11 antibody GDIA 1 antibody GDIA1 antibody GDIR1_HUMAN antibody MGC117248 antibody NPHS8 antibody Rho GDI 1 antibody Rho GDI alpha antibody Rho GDI antibody Rho GDP dissociation inhibitor (GDI) alpha antibody Rho GDP dissociation inhibitor 1 antibody Rho GDP dissociation inhibitor alpha antibody Rho GDP-dissociation inhibitor 1 antibody Rho-GDI alpha antibody RhoGDI 1 antibody RhoGDI alpha antibody RHOGDI antibody RhoGDI1 antibody wu:fa96g11 antibody zgc:55554 antibody zgc:77681 antibody |
|---|---|
| Molecular Weight(MW) | 23kDa |
| Cellular Localization | Cytoplasm.. |
Application
Fig1: Western blot analysis of ARHGDIA on human ARHGDIA recombinant protein using anti-ARHGDIA antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of ARHGDIA on different cell lysates using anti-ARHGDIA antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: HeLa Lane 3: NIH-3T3 Lane 4: C6 Lane 5: K562 Lane 6: COS7
Application
Fig3: ICC staining ARHGDIA (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4:Flow cytometric analysis of Hela cells with ARHGDIA antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human ARHGDIA recombinant protein, Jurkat, HeLa, NIH-3T3, C6, K562, COS7, HepG2, Hela. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:100-1:500 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
