Application
Fig1: Western blot analysis of GCNF on human GCNF recombinant protein using anti- GCNF antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of GCNF on HEK293 (1) and GCNF -hIgGFc transfected HEK293 (2) cell lysate using anti-GCNF antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of GCNF on different cell lysates using anti-GCNF antibody at 1/1,000 dilution. Positive control： Lane1: K562 Lane2: NTERA-2 Lane3: HEK293 Lane4: HUVE-12 Lane5: HeLaApplication
Fig4: ICC staining GCNF (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human cerebellum tissue using anti-GCNF antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-GCNF antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of K562 cells with GCNF antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | GCNF [A10-G8] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GCNF 1 antibody GCNF antibody GCNF1 antibody Germ cell nuclear factor antibody hGCNF antibody hRTR antibody NR 61 antibody NR61 antibody nr6a1 antibody NR6A1_HUMAN antibody Nuclear receptor subfamily 6 group A member 1 antibody Orphan nuclear receptor NR6A1 antibody Retinoic acid receptor related testis associated receptor antibody Retinoid receptor related testis specific receptor antibody Retinoid receptor-related testis-specific receptor antibody RTR antibody |
| Product description | Germ cell nuclear factor (GCNF) is an orphan member of the nuclear receptor gene superfamily that influences neurogenesis and germ cell development. GCNF can homodimerize and bind DNA. GCNF regulates paracrine interaction between the oocyte and somatic cells by regulating the expression of BMP-15 and GDF-9, to affect female fertility. GCNF is present in spermatocytes and round spermatids of adult male mouse testis; northern blot and ribonuclease protection assays have shown GCNF is predominant in the testis. The gene expresses three alternatively spliced transcript variants. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:100-1:500 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | GCNF 1 antibody GCNF antibody GCNF1 antibody Germ cell nuclear factor antibody hGCNF antibody hRTR antibody NR 61 antibody NR61 antibody nr6a1 antibody NR6A1_HUMAN antibody Nuclear receptor subfamily 6 group A member 1 antibody Orphan nuclear receptor NR6A1 antibody Retinoic acid receptor related testis associated receptor antibody Retinoid receptor related testis specific receptor antibody Retinoid receptor-related testis-specific receptor antibody RTR antibody |
|---|---|
| Molecular Weight(MW) | 54 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q15406 |
|---|
Application
Fig1: Western blot analysis of GCNF on human GCNF recombinant protein using anti- GCNF antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of GCNF on HEK293 (1) and GCNF -hIgGFc transfected HEK293 (2) cell lysate using anti-GCNF antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of GCNF on different cell lysates using anti-GCNF antibody at 1/1,000 dilution. Positive control： Lane1: K562 Lane2: NTERA-2 Lane3: HEK293 Lane4: HUVE-12 Lane5: HeLa
Application
Fig4: ICC staining GCNF (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human cerebellum tissue using anti-GCNF antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-GCNF antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of K562 cells with GCNF antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human GCNF recombinant protein, GCNF-hIgGFc transfected HEK293 cell lysate, K562, NTERA-2, HUVE-12, HeLa, HepG2, human cerebellum tissue, human rectum cancer tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:100-1:500 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
