Application
Fig1: Western blot analysis of NCK1 on human NCK1 recombinant protein using anti-NCK1 antibody at 1/1,000 dilution.Application
ApplicationApplication
Fig3: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-NCK1 antibody. Counter stained with hematoxylin.Application
Fig4: Flow cytometric analysis of Jurkat cells with NCK1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | NCK1 [C2-B7] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cytoplasmic protein NCK1 antibody Melanoma Nck protein antibody MGC12668 antibody NCK 1 antibody NCK adaptor protein 1 antibody NCK alpha antibody NCK antibody NCK tyrosine kinase antibody Nck-1 antibody NCK1 antibody NCK1_HUMAN antibody NCKalpha antibody Non catalytic region of tyrosine kinase antibody SH2/SH3 adaptor protein NCK alpha antibody SH2/SH3 adaptor protein NCK-alpha antibody |
| Product description | NCK1 couples tyrosine kinase signaling, including the EGF and PDGF receptor-pathways, to downstream signaling proteins. Specifically, overexpression of Nck1 in NIH/3T3 cells decreases DNA synthesis stimulated by EGF. Furthermore, the SH2 domain of NCK2 inhibits EGF- and PDGF-induced DNA synthesis. The SH3 domain of NCK binds a proline-rich domain on PAK, a known actin cytoskeleton regulator. The NCK protein thus mediates the interaction between PAK and RAC. The NCK2 protein binds human PDGFR-b (Tyr 1009); overexpression of Nck2 inhibits PDGF-induced membrane ruffling and lamellipod formation. Various growth factor receptors, cell surface antigens and adhesion molecules phosphorylate mammalian NCK1 and NCK2. The human NCK1 and NCK2 genes map to chromosomes 3q21 and 2q12, respectively. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:500 IHC:1:50-1:200 FC:1:100-1:200 |
|
| Species Reactivity | HumanMonkey |
| Concentration | 2mg/ml |
| Alternative Names | Cytoplasmic protein NCK1 antibody Melanoma Nck protein antibody MGC12668 antibody NCK 1 antibody NCK adaptor protein 1 antibody NCK alpha antibody NCK antibody NCK tyrosine kinase antibody Nck-1 antibody NCK1 antibody NCK1_HUMAN antibody NCKalpha antibody Non catalytic region of tyrosine kinase antibody SH2/SH3 adaptor protein NCK alpha antibody SH2/SH3 adaptor protein NCK-alpha antibody |
|---|---|
| Molecular Weight(MW) | 43 kDa |
| SwissProt ID | P16333 |
|---|
Application
Fig1: Western blot analysis of NCK1 on human NCK1 recombinant protein using anti-NCK1 antibody at 1/1,000 dilution.
Application
Application
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-NCK1 antibody. Counter stained with hematoxylin.
Application
Fig4: Flow cytometric analysis of Jurkat cells with NCK1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human NCK1 recombinant protein, Hela, Jurkat, HEK293, A431, K562, COS7, human rectum cancer tissue. |
|---|---|
| Application Notes | WB:1:500 IHC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
