Application
Fig1: ICC staining MELK (green) and actin filaments (red) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-MELK antibody. Counter stained with hematoxylin.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-MELK antibody. Counter stained with hematoxylin.Application
Fig4: Flow cytometric analysis of MCF-7 cells with MELK antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | MELK [B7-G2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AI327312 antibody hMELK antibody HPK 38 antibody hPK38 antibody KIAA0175 antibody Likely ortholog of maternal embryonic leucine zipper kinase antibody Maternal embryonic leucine zipper kinase antibody MELK antibody MELK_HUMAN antibody mKIAA0175 antibody MPK38 antibody OTTHUMP00000021377 antibody OTTHUMP00000046113 antibody pEg3 kinase antibody Protein kinase Eg3 antibody Protein kinase PK38 antibody RP23 382O11.1 antibody Tyrosine protein kinase MELK antibody |
| Product description | Maternal embryonic leucine zipper kinase (KIAA0175, HPK38) or MELK, a new member of the Snf1/AMPK family of kinases, encodes a protein with a kinase catalytic domain and a leucine zipper motif consisting of a periodic repetition of leucine residues at every seventh residue located within the N-terminal catalytic domain. This motif has been observed in myriad DNA-binding proteins and is presumed to be involved in protein-DNA interactions, and potentially protein-protein interactions. Research predicts that the gene product of MELK plays a role in the signal transduction events in the egg and early embryo. Mouse and human MELK proteins share 95% sequence identity in the kinase domain and northern blot analysis in mouse indicates that MELK expression is restricted to spermatogonia in the testis and to oocytes in the ovary. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | ICC/IFICCIHCFC |
| ICC:1:200-1:1000 IHC:1:200-1:1,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | AI327312 antibody hMELK antibody HPK 38 antibody hPK38 antibody KIAA0175 antibody Likely ortholog of maternal embryonic leucine zipper kinase antibody Maternal embryonic leucine zipper kinase antibody MELK antibody MELK_HUMAN antibody mKIAA0175 antibody MPK38 antibody OTTHUMP00000021377 antibody OTTHUMP00000046113 antibody pEg3 kinase antibody Protein kinase Eg3 antibody Protein kinase PK38 antibody RP23 382O11.1 antibody Tyrosine protein kinase MELK antibody |
|---|---|
| Molecular Weight(MW) | 75kDa |
| Cellular Localization | Cell membrane. |
| SwissProt ID | Q14680 |
|---|
Application
Fig1: ICC staining MELK (green) and actin filaments (red) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-MELK antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-MELK antibody. Counter stained with hematoxylin.
Application
Fig4: Flow cytometric analysis of MCF-7 cells with MELK antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | HepG2, human liver cancer tissue, human rectum cancer tissue, MCF-7. |
|---|---|
| Application Notes | ICC:1:200-1:1000 IHC:1:200-1:1,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
