Application
Fig1: Western blot analysis of PLK1 on human PLK1 recombinant protein using anti-PLK1 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of PLK1 on K562 (1) and Raji (2) cell lysate using anti-PLK1 antibody at 1/1,000 dilution.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-PLK1 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-PLK1 antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of NIH-3T3 cells with PLK1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | PLK1 [G10-C11] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cell cycle regulated protein kinase antibody PLK 1 antibody PLK antibody PLK-1 antibody plk1 antibody PLK1_HUMAN antibody Polo like kinase 1 antibody Polo-like kinase 1 antibody Serine/threonine protein kinase 13 antibody Serine/threonine protein kinase PLK1 antibody Serine/threonine-protein kinase 13 antibody Serine/threonine-protein kinase PLK1 antibody STPK 13 antibody STPK13 antibody |
| Product description | Plk (for polo-like kinase) encodes a serine/threonine kinase that is closely related to polo and CDC5, genes that are required for passage through mitosis in Drosophila and Saccharomyces, respectively. Polo and CDC5 both code for proteins that are involved in regulating the function of the mitotic spindle. Plk protein accumulates in the cell during the S and G2 phases of the cell cycle and both protein content and catalytic activity peak at the onset of mitosis, followed by a rapid reduction after mitosis. Plk expression is detectable in mitotically active tissues such as colon and placenta, as well as in tumors of various origins. It has also been suggested that Plk may serve as a marker of cell proliferation. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:500-1:1,000 IHC:1:50-1:200 FC:1:100-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Alternative Names | Cell cycle regulated protein kinase antibody PLK 1 antibody PLK antibody PLK-1 antibody plk1 antibody PLK1_HUMAN antibody Polo like kinase 1 antibody Polo-like kinase 1 antibody Serine/threonine protein kinase 13 antibody Serine/threonine protein kinase PLK1 antibody Serine/threonine-protein kinase 13 antibody Serine/threonine-protein kinase PLK1 antibody STPK 13 antibody STPK13 antibody |
|---|---|
| Molecular Weight(MW) | 68 kDa |
| Cellular Localization | Nucleus. |
Application
Fig1: Western blot analysis of PLK1 on human PLK1 recombinant protein using anti-PLK1 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of PLK1 on K562 (1) and Raji (2) cell lysate using anti-PLK1 antibody at 1/1,000 dilution.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-PLK1 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-PLK1 antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of NIH-3T3 cells with PLK1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human PLK1 recombinant protein, K562, Raji, human stomach cancer tissue, human rectum cancer tissue, NIH-3T3. |
|---|---|
| Application Notes | WB:1:500-1:1,000 IHC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
