Application
Fig1: Western blot analysis of EEF2 on different cell lysates using anti-EEF2 antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 Lane 2: Hela Lane 3: HEK293 Lane 4: A431Application
Fig2: ICC staining EEF2 (green) and actin filaments (red) in 3T3-L1 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-EEF2 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney cancer tissue using anti-EEF2 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-EEF2 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-EEF2 antibody. Counter stained with hematoxylin.| Product Name | EEF2 [H1-B6] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EEF 2 antibody Eef2 antibody EF-2 antibody EF2 antibody EF2_HUMAN antibody Elongation factor 2 antibody Eukaryotic translation elongation factor 2 antibody Polypeptidyl tRNA translocase antibody SCA26 antibody |
| Product description | Two elongation factors (EF) EF-Tu and EF-2 participate in the elongation phase during protein biosynthesis on the ribosome and their functional cycles depend on GTP binding and its hydrolysis. EF-Tu (also designated mitochondrial precursor p43) and EF-2 are multidomain GTPases with essential functions in translation, and they both bind to the same site on the ribosome where their low intrinsic GTPase activities are strongly stimulated. EF-Tu plays a central role in the fast and accurate delivery of aminoacyl-tRNAs to the translating ribosome. In addition, EF-Tu protects the aminoester bond against hydrolysis until a correct match between the codon on mRNA and the anticodon on tRNA can be achieved. EF-2 supports the translocation of tRNAs and of mRNAs on the ribosome so that a new codon can be exposed for decoding. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHC |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | EEF 2 antibody Eef2 antibody EF-2 antibody EF2 antibody EF2_HUMAN antibody Elongation factor 2 antibody Eukaryotic translation elongation factor 2 antibody Polypeptidyl tRNA translocase antibody SCA26 antibody |
|---|---|
| Molecular Weight(MW) | 95 kDa |
| Cellular Localization | Cytoplasm. |
Application
Fig1: Western blot analysis of EEF2 on different cell lysates using anti-EEF2 antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 Lane 2: Hela Lane 3: HEK293 Lane 4: A431
Application
Fig2: ICC staining EEF2 (green) and actin filaments (red) in 3T3-L1 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-EEF2 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney cancer tissue using anti-EEF2 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-EEF2 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-EEF2 antibody. Counter stained with hematoxylin.| Positive Control | Hela, HepG2, HEK293, A431, 3T3-L1, human liver cancer tissue, human kidney cancer tissue, human prostate cancer tissue, human tonsil tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
