Application
Fig1: Western blot analysis of PBK on human PBK recombinant protein using anti-PBK antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of PBK on A431 cell lysate using anti-PBK antibody at 1/1,000 dilution.Application
Fig3: ICC staining PBK (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-PBK antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PBK antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of Hela cells with PBK antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | PBK [E11-C8] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cancer/testis antigen 84 antibody CT84 antibody Epididymis luminal protein 164 antibody FLJ14385 antibody HEL164 antibody Lymphokine activated killer T cell originated protein kinase antibody Lymphokine-activated killer T-cell-originated protein kinase antibody MAPKK like protein kinase antibody MAPKK-like protein kinase antibody Nori 3 antibody Nori-3 antibody Nori3 antibody PBK antibody PDZ binding kinase antibody PDZ-binding kinase antibody Serine/threonine protein kinase antibody Spermatogenesis related protein kinase antibody Spermatogenesis-related protein kinase antibody SPK antibody T LAK cell originated protein kinase antibody T-LAK cell-originated protein kinase antibody TOPK antibody TOPK_HUMAN antibody |
| Product description | Protein kinases comprise a large group of encoded factors that regulate cellular processes by catalyzing the transfer of a phosphate group to a hydroxyl acceptor in serine, threonine or tyrosine residues. Kinases are capable of influencing the oncogenic potential of cell sytems at the level of oncoprotein or tumor suppressor protein phosphorylation states. Human PDZ-binding kinase, known as PBK, is a 322 amino acid, T/SXV motif-containing serine/threonine kinase that is abuntant in placenta and absent from adult brain tissue. A PDZ domain in the tumor suppressor protein Dlg can coordinate with the T/SXV motif of PBK. The cell cycle checkpoint kinase Cdc2/cyclin B is an upstream effector of PBK that can phosphorylate and activate PBK. Active PBK may associate with PDZ-containing proteins and influence cell cycle control or cellular proliferation. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500 ICC:1:100-1:500 IHC:1:100-1:500 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | Cancer/testis antigen 84 antibody CT84 antibody Epididymis luminal protein 164 antibody FLJ14385 antibody HEL164 antibody Lymphokine activated killer T cell originated protein kinase antibody Lymphokine-activated killer T-cell-originated protein kinase antibody MAPKK like protein kinase antibody MAPKK-like protein kinase antibody Nori 3 antibody Nori-3 antibody Nori3 antibody PBK antibody PDZ binding kinase antibody PDZ-binding kinase antibody Serine/threonine protein kinase antibody Spermatogenesis related protein kinase antibody Spermatogenesis-related protein kinase antibody SPK antibody T LAK cell originated protein kinase antibody T-LAK cell-originated protein kinase antibody TOPK antibody TOPK_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 36 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q96KB5 |
|---|
Application
Fig1: Western blot analysis of PBK on human PBK recombinant protein using anti-PBK antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of PBK on A431 cell lysate using anti-PBK antibody at 1/1,000 dilution.
Application
Fig3: ICC staining PBK (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-PBK antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PBK antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of Hela cells with PBK antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human PBK recombinant protein, A431, Hela, human ovarian cancer tissue, human colon cancer tissue. |
|---|---|
| Application Notes | WB:1:500 ICC:1:100-1:500 IHC:1:100-1:500 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
