Application
Fig1: Western blot analysis of CCL2 on human CCL2 recombinant protein using anti-CCL2 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of CCL2 on different cell lysates using anti-CCL2 antibody at 1/1,000 dilution. Positive control: Lane 1: A549 Lane 2: HELA Lane 3: Raw264.7 Lane 4: L1210 Lane 5: C6 Lane 6: Cos7Application
Fig3: ICC staining CCL2 (green) and actin filaments (red) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
ApplicationApplication
Fig5: Flow cytometric analysis of A549 cells with CCL2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | CCL2 [E11-D8] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:1,000 ICC:1:100-1:500 IHC:1:100-1:500 FC:1:100-1:200 |
|
| Species Reactivity | HumanMouseMonkey |
| Concentration | 2mg/ml |
| Molecular Weight(MW) | 15 kDa |
|---|---|
| Cellular Localization | Secreted. |
Application
Fig1: Western blot analysis of CCL2 on human CCL2 recombinant protein using anti-CCL2 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of CCL2 on different cell lysates using anti-CCL2 antibody at 1/1,000 dilution. Positive control: Lane 1: A549 Lane 2: HELA Lane 3: Raw264.7 Lane 4: L1210 Lane 5: C6 Lane 6: Cos7
Application
Fig3: ICC staining CCL2 (green) and actin filaments (red) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Application
Application
Fig5: Flow cytometric analysis of A549 cells with CCL2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human CCL2 recombinant protein, Hela, A549, Raw264.7, L1210, C6, COS-7, liver cancer tissue, HepG2. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:100-1:500 IHC:1:100-1:500 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
