Application
Fig1: Western blot analysis of TWIST1 on human TWIST1 recombinant protein using anti-TWIST1 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of TWIST1 on HEK293 (1) and TWIST1-hIgGFc transfected HEK293 (2) cell lysate using anti-TWIST1 antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of TWIST1 on different cell lysates using anti-TWIST1 antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3 Lane 2: JURKAT Lane 3: HELA Lane 4: A549 Lane 5: RAJI Lane 6: OCM-1Application
Fig4: ICC staining TWIST1 (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded cervical cancer tissue using anti-TWIST1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded colon cancer tissue using anti-TWIST1 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of Hela cells with TWIST1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | TWIST1 [E6-F4] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ACS3 antibody B-HLH DNA binding protein antibody bHLHa38 antibody BPES2 antibody BPES3 antibody Class A basic helix-loop-helix protein 38 antibody CRS antibody CRS1 antibody CSO antibody H-twist antibody OTTHUMP00000116043 antibody SCS antibody Twist basic helix loop helix transcription factor 1 antibody Twist family bHLH transcription factor 1 antibody Twist homolog 1 (Drosophila) antibody Twist homolog 1 antibody TWIST homolog of drosophila antibody Twist related protein 1 antibody Twist-related protein 1 antibody TWIST1 antibody TWST1_HUMAN antibody |
| Product description | Members of the myogenic determination family are basic helix-loop-helix (bHLH) proteins that can be separated into two classes. Class A proteins include the ubiquitously expressed E-box binding factors E12/E47, ITF2 and HEB (BETA1 or HTF4). Class B proteins such as MyoD, myogenin and NeuroD (BETA2) are transiently expressed and exhibit a much more limited tissue distribution. Class A proteins heterodimerize with class B proteins to activate DNA transcription. Working in opposition to these positively acting factors are a specialized group of proteins that function as dominant negative regulators. The novel basic helix-loop-helix (bHLH) transcription factor twist is a putative regulator of mesodermal differentiation and myogenesis. Twist is expressed throughout the epithelial somite but not in the myotome. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:100-1:500 FC:1:100-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Alternative Names | ACS3 antibody B-HLH DNA binding protein antibody bHLHa38 antibody BPES2 antibody BPES3 antibody Class A basic helix-loop-helix protein 38 antibody CRS antibody CRS1 antibody CSO antibody H-twist antibody OTTHUMP00000116043 antibody SCS antibody Twist basic helix loop helix transcription factor 1 antibody Twist family bHLH transcription factor 1 antibody Twist homolog 1 (Drosophila) antibody Twist homolog 1 antibody TWIST homolog of drosophila antibody Twist related protein 1 antibody Twist-related protein 1 antibody TWIST1 antibody TWST1_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 25 kDa |
| Cellular Localization | Nucleus. |
Application
Fig1: Western blot analysis of TWIST1 on human TWIST1 recombinant protein using anti-TWIST1 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of TWIST1 on HEK293 (1) and TWIST1-hIgGFc transfected HEK293 (2) cell lysate using anti-TWIST1 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of TWIST1 on different cell lysates using anti-TWIST1 antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3 Lane 2: JURKAT Lane 3: HELA Lane 4: A549 Lane 5: RAJI Lane 6: OCM-1
Application
Fig4: ICC staining TWIST1 (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded cervical cancer tissue using anti-TWIST1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded colon cancer tissue using anti-TWIST1 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of Hela cells with TWIST1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human TWIST1 recombinant protein, TWIST1-hIgGFc transfected HEK293 cell lysate, NIH/3T3, JURKAT, HELA, A549, RAJI, OCM-1, Hela, cervical cancer tissue, colon cancer tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:100-1:500 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
