Application
Fig1: Western blot analysis of HSPB2 on human HSPB2 recombinant protein using anti-HSPB2 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of HSPB2 on HEK293 (1) and HSPB2-hIgGFc transfected HEK293 (2) cell lysate using anti-HSPB2 antibody at 1/1,000 dilution.Application
Fig3: Flow cytometric analysis of A549 cells with HSPB2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | HSPB2 [E2-B9] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DMPK-binding protein antibody Heat shock 27kD protein 2 antibody Heat shock 27kDa protein 2 antibody Heat shock protein beta-2 antibody Heat-shock protein beta-2 antibody Hs.78846 antibody HSP27 antibody HspB2 antibody HSPB2_HUMAN antibody LOH11CR1K antibody MGC133245 antibody MKBP antibody |
| Product description | The heat shock proteins (HSPs) comprise a group of highly conserved, abundantly expressed proteins with diverse functions, including the assembly and sequestering of multi-protein complexes, transportation of nascent polypeptide chains across cellular membranes and regulation of protein folding. HSPB2 (heat shock 27 kDa protein 2), also known as HSP 27 or MKBP, is a 182 amino acid protein that belongs to the heat shock protein family and is expressed preferentially in heart and skeletal muscle. Localized to mitochondria, HSPB2 functions as an ATP-dependent chaperone protein that plays a role in the refolding of denatured proteins and may also interact with the Actin cytoskeleton and prevent apoptotic cell death. HSPB2 is abundantly expressed in several cancer cell lines, suggesting that HSPB2 may be an important factor in tumor transformation and metastasis. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | DMPK-binding protein antibody Heat shock 27kD protein 2 antibody Heat shock 27kDa protein 2 antibody Heat shock protein beta-2 antibody Heat-shock protein beta-2 antibody Hs.78846 antibody HSP27 antibody HspB2 antibody HSPB2_HUMAN antibody LOH11CR1K antibody MGC133245 antibody MKBP antibody |
|---|---|
| Molecular Weight(MW) | 20 kDa |
| Cellular Localization | Cytoplasm. Nucleus. |
| SwissProt ID | Q16082 |
|---|
Application
Fig1: Western blot analysis of HSPB2 on human HSPB2 recombinant protein using anti-HSPB2 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of HSPB2 on HEK293 (1) and HSPB2-hIgGFc transfected HEK293 (2) cell lysate using anti-HSPB2 antibody at 1/1,000 dilution.
Application
Fig3: Flow cytometric analysis of A549 cells with HSPB2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human HSPB2 recombinant protein, HSPB2-hIgGFc transfected HEK293 cell lysate, A549. |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
