Application
Fig1: Western blot analysis of PPM1A on human PPM1A recombinant protein using anti-PPM1A antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of PPM1A on HEK293 (1) and PPM1A-hIgGFc transfected HEK293 (2) cell lysate using anti-PPM1A antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of PPM1A on different lysates using anti-PPM1A antibody at 1/1,000 dilution. Positive control： Lane1: Jurkat Lane2: Jurkat Lane3: A431 Lane4: HeLa Lane5: HEK293 Lane6: Raji Lane7: MCF-7 Lane8: COS7Application
Fig4: Flow cytometric analysis of Hela cells with PPM1A antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | PPM1A [H6-F12] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Protein phosphatase 2C isoform alpha antibody EC 3.1.3.16 antibody FLJ42306 antibody IA antibody MGC9201 antibody Mpp alpha antibody PP2C alpha antibody PP2C-alpha antibody PP2CA antibody PP2Calpha antibody PPM 1A antibody PPM1A antibody PPM1A_HUMAN antibody PPPM1A antibody Protein phosphatase 1A (formerly 2C) magnesium dependent alpha isoform antibody Protein phosphatase 1A antibody Protein phosphatase 1A magnesium dependent alpha antibody Protein phosphatase 2C alpha antibody Protein phosphatase 2C alpha isoform antibody Protein phosphatase 2C isoform alpha antibody Protein phosphatase IA antibody Protein phosphatase, Mg2+/Mn2+ dependent, 1A antibody |
| Product description | Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling. Dephosphorylates PRKAA1 and PRKAA2. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | HumanMonkey |
| Concentration | 2mg/ml |
| Alternative Names | Protein phosphatase 2C isoform alpha antibody
EC 3.1.3.16 antibody
FLJ42306 antibody
IA antibody
MGC9201 antibody
Mpp alpha antibody
PP2C alpha antibody
PP2C-alpha antibody
PP2CA antibody
PP2Calpha antibody
PPM 1A antibody
PPM1A antibody
PPM1A_HUMAN antibody
PPPM1A antibody
Protein phosphatase 1A (formerly 2C) magnesium dependent alpha isoform antibody
Protein phosphatase 1A antibody
Protein phosphatase 1A magnesium dependent alpha antibody
Protein phosphatase 2C alpha antibody
Protein phosphatase 2C alpha isoform antibody
Protein phosphatase 2C isoform alpha antibody
Protein phosphatase IA antibody
Protein phosphatase Mg2+/Mn2+ dependent 1A antibody |
|---|---|
| Molecular Weight(MW) | 43 kDa |
| Cellular Localization | Cytosol, Nucleus. |
| SwissProt ID | P35813 |
|---|
Application
Fig1: Western blot analysis of PPM1A on human PPM1A recombinant protein using anti-PPM1A antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of PPM1A on HEK293 (1) and PPM1A-hIgGFc transfected HEK293 (2) cell lysate using anti-PPM1A antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of PPM1A on different lysates using anti-PPM1A antibody at 1/1,000 dilution. Positive control： Lane1: Jurkat Lane2: Jurkat Lane3: A431 Lane4: HeLa Lane5: HEK293 Lane6: Raji Lane7: MCF-7 Lane8: COS7
Application
Fig4: Flow cytometric analysis of Hela cells with PPM1A antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human PPM1A recombinant protein , PPM1A-hIgGFc transfected HEK293 cell lysate, Jurkat, A431, HeLa, HEK293, Raji, MCF-7, COS7. |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
