Application
Western blot analysis of CHRNA10 against human CHRNA10 (AA: extra 25-237) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-33, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of CHRNA10 against HEK293 (1) and CHRNA10 (AA: extra 25-237)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-33, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of CHRNA10 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-33, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | CHRNA10 [6C10E8] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Acetylcholine receptor, neuronal nicotinic, alpha-10 subunit antibody ACH10_HUMAN antibody Alpha 10 nAChR antibody Cholinergic receptor nicotinic alpha 10 antibody Cholinergic receptor, neuronal nicotinic, alpha polypeptide 10 antibody Cholinergic receptor, nicotinic, alpha polypeptide 10 antibody CHRNA 10 antibody CHRNA10 antibody NACHR alpha 10 antibody NACHR alpha-10 antibody NACHRA10 antibody Neuronal acetylcholine receptor protein subunit alpha 10 antibody Neuronal acetylcholine receptor subunit alpha-10 antibody Nicotinic acetylcholine receptor subunit alpha 10 antibody Nicotinic acetylcholine receptor subunit alpha-10 antibody OTTHUMP00000013620 antibody |
| Product description | Neuronal acetylcholine receptor subunit alpha-10, also known as nAChRα10 and cholinergic receptor nicotinic alpha 10, is a protein that in humans is encoded by the CHRNA10 gene.[5] The protein encoded by this gene is a subunit of certain nicotinic acetylcholine receptors (nAchR). This nAchR subunit is required for the normal function of the olivocochlear system which is part of the auditory system. Furthermore, selective block of α9α10 nicotinic acetylcholine receptors by the conotoxin RgIA has been shown to be analgesic in an animal model of nerve injury pain. CHRNA10 (Cholinergic Receptor Nicotinic Alpha 10 Subunit) is a Protein Coding gene. Among its related pathways are Peptide ligand-binding receptors and CREB Pathway. GO annotations related to this gene include receptor binding and extracellular ligand-gated ion channel activity. An important paralog of this gene is CHRNA9. |
| Immunogen | Purified recombinant fragment of human CHRNA10 (AA: extra 25-237) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | Acetylcholine receptor neuronal nicotinic alpha-10 subunit antibody ACH10_HUMAN antibody Alpha 10 nAChR antibody Cholinergic receptor nicotinic alpha 10 antibody Cholinergic receptor neuronal nicotinic alpha polypeptide 10 antibody Cholinergic receptor nicotinic alpha polypeptide 10 antibody CHRNA 10 antibody CHRNA10 antibody NACHR alpha 10 antibody NACHR alpha-10 antibody NACHRA10 antibody Neuronal acetylcholine receptor protein subunit alpha 10 antibody Neuronal acetylcholine receptor subunit alpha-10 antibody Nicotinic acetylcholine receptor subunit alpha 10 antibody Nicotinic acetylcholine receptor subunit alpha-10 antibody OTTHUMP00000013620 antibody |
|---|---|
| Molecular Weight(MW) | 49.7kDa |
| Cellular Localization | Cell junction. Cell membrane. |
| SwissProt ID | Q9GZZ6 |
|---|
Application
Western blot analysis of CHRNA10 against human CHRNA10 (AA: extra 25-237) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-33, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of CHRNA10 against HEK293 (1) and CHRNA10 (AA: extra 25-237)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-33, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of CHRNA10 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-33, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | Hela cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
