Application
Western blot analysis of BCL9L against human BCL9L (AA: 606-751) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-37, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of BCL9L against HEK293 (1) and BCL9L (AA: 606-751)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-37, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Immunohistochemical analysis of paraffin-embedded cervical cancer tissue using anti-BCL9L antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1710-37, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | BCL9L [3B9C1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | B-cell CLL antibody B-cell CLL/lymphoma 9-like protein antibody B-cell lymphoma 9-like protein antibody B9L antibody BCL9-2 antibody BCL9-like protein antibody BCL9-related beta-catenin-binding protein antibody Bcl9l antibody BCL9L_HUMAN antibody DLNB11 antibody Nuclear co factor of beta catenin signalling antibody Protein BCL9-2 antibody |
| Product description | BCL9L (B-Cell CLL/Lymphoma 9-Like) is a Protein Coding gene. Among its related pathways are Wnt Signaling Pathways: beta-Catenin-dependent Wnt Signaling and Signaling by Wnt. GO annotations related to this gene include transcription coactivator activity and beta-catenin binding. An important paralog of this gene is BCL9. |
| Immunogen | Purified recombinant fragment of human BCL9L (AA: 606-751) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:500-1:2,000 IHC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | B-cell CLL antibody B-cell CLL/lymphoma 9-like protein antibody B-cell lymphoma 9-like protein antibody B9L antibody BCL9-2 antibody BCL9-like protein antibody BCL9-related beta-catenin-binding protein antibody Bcl9l antibody BCL9L_HUMAN antibody DLNB11 antibody Nuclear co factor of beta catenin signalling antibody Protein BCL9-2 antibody |
|---|---|
| Molecular Weight(MW) | 157kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q86UU0 |
|---|
Application
Western blot analysis of BCL9L against human BCL9L (AA: 606-751) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-37, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of BCL9L against HEK293 (1) and BCL9L (AA: 606-751)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-37, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Immunohistochemical analysis of paraffin-embedded cervical cancer tissue using anti-BCL9L antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1710-37, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Positive Control | Hela cells 、cervical cancer tissues |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
