Application
Western blot analysis of ADORA2A against human ADORA2A (AA: 274-412) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-38, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of ADORA2A against HEK293 (1) and ADORA2A (AA: 274-412)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-38, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Immunocytochemistry staining of ADORA2A in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1710-38, 1/100) for 1 hour at room temperature, washed with PBS. Alexa FluorApplication
Flow cytometric analysis of ADORA2A was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-38, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | ADORA2A [6A6G11] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | A2AAR antibody A2aR antibody AA2AR_HUMAN antibody ADENO antibody Adenosine A2 receptor antibody Adenosine A2a receptor antibody Adenosine receptor A2a antibody Adenosine receptor subtype A2a antibody ADORA 2 antibody ADORA2 antibody ADORA2A antibody hA2aR antibody RDC 8 antibody RDC8 antibody |
| Product description | This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor (GPCR) superfamily, which is subdivided into classes and subtypes. The receptors are seven-pass transmembrane proteins that respond to extracellular cues and activate intracellular signal transduction pathways. This protein, an adenosine receptor of A2A subtype, uses adenosine as the preferred endogenous agonist and preferentially interacts with the G(s) and G(olf) family of G proteins to increase intracellular cAMP levels. It plays an important role in many biological functions, such as cardiac rhythm and circulation, cerebral and renal blood flow, immune function, pain regulation, and sleep. It has been implicated in pathophysiological conditions such as inflammatory diseases and neurodegenerative disorders. Alternative splicing results in multiple transcript variants. A read-through transcript composed of the upstream SPECC1L (sperm antigen with calponin homology and coiled-coil domains 1-like) and ADORA2A (adenosine A2a receptor) gene sequence has been identified, but it is thought to be non-coding. |
| Immunogen | Purified recombinant fragment of human ADORA2A (AA: 274-412) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:500-1:2,000 ICC:1：50-1:200 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | A2AAR antibody A2aR antibody AA2AR_HUMAN antibody ADENO antibody Adenosine A2 receptor antibody Adenosine A2a receptor antibody Adenosine receptor A2a antibody Adenosine receptor subtype A2a antibody ADORA 2 antibody ADORA2 antibody ADORA2A antibody hA2aR antibody RDC 8 antibody RDC8 antibody |
|---|---|
| Molecular Weight(MW) | 44.7kDa |
| Cellular Localization | Cell membrane. |
| SwissProt ID | P29274 |
|---|
Application
Western blot analysis of ADORA2A against human ADORA2A (AA: 274-412) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-38, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of ADORA2A against HEK293 (1) and ADORA2A (AA: 274-412)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-38, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Immunocytochemistry staining of ADORA2A in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1710-38, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor
Application
Flow cytometric analysis of ADORA2A was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-38, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | Hela cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1：50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
