Application
Western blot analysis of BATF against human BATF (AA: 1-126) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-39, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of BATF against HEK293 (1) and BATF (AA: 1-126)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-39, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.| Product Name | BATF [7C8F3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Activating transcription factor B antibody B ATF antibody B-ATF antibody B-cell-activating transcription factor antibody Basic leucine zipper transcription factor like antibody Basic leucine zipper transcriptional factor ATF like antibody Basic leucine zipper transcriptional factor ATF-like antibody Batf antibody BATF_HUMAN antibody BATF1 antibody SF HT activated gene 2 protein antibody SF-HT-activated gene 2 protein antibody SFA 2 antibody SFA-2 antibody SFA2 antibody |
| Product description | Basic leucine zipper transcription factor, ATF-like, also known as BATF, is a protein which in humans is encoded by the BATF gene. The protein encoded by this gene is a nuclear basic leucine zipper (bZIP) protein that belongs to the AP-1/ATF superfamily of transcription factors. The leucine zipper of this protein mediates dimerization with members of the Jun family of proteins. This protein is thought to be a negative regulator of AP-1/ATF transcriptional events. Mice without the BATF gene (BATF knockout mice) lacked a type of inflammatory immune cell (Th17) and were resistant to conditions that normally induces an autoimmune condition similar to multiple sclerosis. |
| Immunogen | Purified recombinant fragment of human BATF (AA:1-126) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500-1:2,000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | Activating transcription factor B antibody B ATF antibody B-ATF antibody B-cell-activating transcription factor antibody Basic leucine zipper transcription factor like antibody Basic leucine zipper transcriptional factor ATF like antibody Basic leucine zipper transcriptional factor ATF-like antibody Batf antibody BATF_HUMAN antibody BATF1 antibody SF HT activated gene 2 protein antibody SF-HT-activated gene 2 protein antibody SFA 2 antibody SFA-2 antibody SFA2 antibody |
|---|---|
| Molecular Weight(MW) | 14.1kDa |
| Cellular Localization | Nucleus. Cytoplasm. Present in the nucleus and cytoplasm, but shows increased nuclear translocation after activation of T-cells. |
| SwissProt ID | Q16520 |
|---|
Application
Western blot analysis of BATF against human BATF (AA: 1-126) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-39, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of BATF against HEK293 (1) and BATF (AA: 1-126)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-39, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.| Positive Control | Hela cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
