Application
Western blot analysis of ASF1A against human ASF1A (AA: 1-204) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-40, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of ASF1A against HEK293 (1) and ASF1A (AA: 1-204)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-40, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of ASF1A was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-40, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | ASF1A [2A5F8] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Anti silencing function 1A antibody Anti-silencing function protein 1 homolog A antibody ASF1 anti silencing function 1 homolog A (S. cerevisiae) antibody ASF1 anti silencing function 1 homolog A antibody asf1a antibody ASF1A_HUMAN antibody CCG1 interacting factor A antibody CCG1-interacting factor A antibody CGI 98 antibody CIA antibody hAsf1 antibody hAsf1a antibody hCIA antibody Histone chaperone ASF1A antibody HSPC146 antibody |
| Product description | This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The protein is a key component of a histone donor complex that functions in nucleosome assembly. It interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair. |
| Immunogen | Purified recombinant fragment of human ASF1A (AA: 1-204) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | Anti silencing function 1A antibody Anti-silencing function protein 1 homolog A antibody ASF1 anti silencing function 1 homolog A (S. cerevisiae) antibody ASF1 anti silencing function 1 homolog A antibody asf1a antibody ASF1A_HUMAN antibody CCG1 interacting factor A antibody CCG1-interacting factor A antibody CGI 98 antibody CIA antibody hAsf1 antibody hAsf1a antibody hCIA antibody Histone chaperone ASF1A antibody HSPC146 antibody |
|---|---|
| Molecular Weight(MW) | 23kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q9Y294 |
|---|
Application
Western blot analysis of ASF1A against human ASF1A (AA: 1-204) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-40, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of ASF1A against HEK293 (1) and ASF1A (AA: 1-204)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-40, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of ASF1A was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-40, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | Hela cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
