Application
Western blot analysis of ADAP1 against human ADAP1 (AA: 240-370) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-41, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of ADAP1 against HEK293 (1) and ADAP1 (AA: 240-370)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-41, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of ADAP1 against C6 (1), A549 (2), and HepG2 (3) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-41, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of ADAP1 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-41, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | ADAP1 [3E2D9] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ArfGAP with dual PH domains 1 antibody CENTA 1 antibody CENTA1 antibody Centaurin alpha antibody GCS1L antibody p42IP4 antibody Putative MAPK activating protein PM25 antibody |
| Product description | ADAP1 (ArfGAP With Dual PH Domains 1) is a Protein Coding gene. Among its related pathways are Class I PI3K signaling events and B Cell Receptor Signaling Pathway (sino). GO annotations related to this gene include GTPase activator activity and inositol 1,3,4,5 tetrakisphosphate binding. An important paralog of this gene is ADAP2. |
| Immunogen | Purified recombinant fragment of human ADAP1 (AA: 240-370) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/mL |
| Alternative Names | ArfGAP with dual PH domains 1 antibody CENTA 1 antibody CENTA1 antibody Centaurin alpha antibody GCS1L antibody p42IP4 antibody Putative MAPK activating protein PM25 antibody |
|---|---|
| Molecular Weight(MW) | 43.4kDa |
| Cellular Localization | Cytoplasmic, Nuclear and Plasma membrane |
| SwissProt ID | O75689 |
|---|
Application
Western blot analysis of ADAP1 against human ADAP1 (AA: 240-370) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-41, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of ADAP1 against HEK293 (1) and ADAP1 (AA: 240-370)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-41, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of ADAP1 against C6 (1), A549 (2), and HepG2 (3) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-41, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of ADAP1 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-41, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | C6, A549, and HepG2 cell、K562 cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
