Application
Western blot analysis of TNFSF11 against human TNFSF11 (AA: 74-308) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-42, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of TNFSF11 against HEK293 (1) and TNFSF11 (AA: 74-308)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-42, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of TNFSF11 against COS7 (1), Hela (2), U937 (3), HL-60 (4), Raji (5), Ramos (6), Jurkat (7), and SW480 (8) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-42, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Immunocytochemistry staining of TNFSF11 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1710-42, 1/100) for 1 hour at room temperature, washed with PBS. Alexa FluorApplication
Immunohistochemical analysis of paraffin-embedded bladder cancer tissue using anti-TNFSF11 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1710-42, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Application
Flow cytometric analysis of TNFSF11 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-42, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | TNFSF11 [8A7B9] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD254 antibody hRANKL2 antibody ODF antibody OPGL antibody OPTB2 antibody Osteoclast differentiation factor antibody Osteoprotegerin ligand antibody RANKL antibody Receptor activator of nuclear factor kappa B ligand antibody Receptor activator of nuclear factor kappa-B ligand antibody sOdf antibody TNF related activation induced cytokine antibody TNF-related activation-induced cytokine antibody TNF11_HUMAN antibody TNFSF 11 antibody Tnfsf11 antibody TRANCE antibody Tumor necrosis factor (ligand) superfamily member 11 antibody Tumor necrosis factor ligand superfamily member 11 antibody Tumor necrosis factor ligand superfamily member 11, soluble form antibody |
| Product description | This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found. |
| Immunogen | Purified recombinant fragment of human TNFSF11 (AA: 74-308) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCICCFC |
| WB:1:500-1:2,000 IHC:1：50-1:200 ICC:1：50-1:200 FC:1:100-1:200 |
|
| Species Reactivity | HumanMonkey |
| Concentration | 1mg/mL |
| Alternative Names | CD254 antibody
hRANKL2 antibody
ODF antibody
OPGL antibody
OPTB2 antibody
Osteoclast differentiation factor antibody
Osteoprotegerin ligand antibody
RANKL antibody
Receptor activator of nuclear factor kappa B ligand antibody
Receptor activator of nuclear factor kappa-B ligand antibody
sOdf antibody
TNF related activation induced cytokine antibody
TNF-related activation-induced cytokine antibody
TNF11_HUMAN antibody
TNFSF 11 antibody
Tnfsf11 antibody
TRANCE antibody
Tumor necrosis factor (ligand) superfamily member 11 antibody
Tumor necrosis factor ligand superfamily member 11 antibody
Tumor necrosis factor ligand superfamily member 11 soluble form antibody |
|---|---|
| Molecular Weight(MW) | 35.5kDa |
| Cellular Localization | Cytoplasm; Secreted and Cell membrane. |
| SwissProt ID | O14788 |
|---|
Application
Western blot analysis of TNFSF11 against human TNFSF11 (AA: 74-308) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-42, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of TNFSF11 against HEK293 (1) and TNFSF11 (AA: 74-308)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-42, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of TNFSF11 against COS7 (1), Hela (2), U937 (3), HL-60 (4), Raji (5), Ramos (6), Jurkat (7), and SW480 (8) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-42, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Immunocytochemistry staining of TNFSF11 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1710-42, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor
Application
Immunohistochemical analysis of paraffin-embedded bladder cancer tissue using anti-TNFSF11 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1710-42, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Flow cytometric analysis of TNFSF11 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-42, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | COS7, Hela, U937, HL-60, Raji, Ramos, Jurkat, and SW480 cell 、Hela cells 、bladder cancer tissues |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1：50-1:200 ICC:1：50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
