Application
Western blot analysis of AQP2 against human AQP2 (AA: 149-271) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-44, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of AQP2 against HEK293 (1) and AQP2 (AA: 149-271)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-44, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of AQP2 against K562 (1), HeLa (2), HCT116 (3), and SW480 (4) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-44, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.| Product Name | AQP2 [7H8B5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ADH water channel antibody AQP 2 antibody AQP CD antibody AQP-2 antibody AQP-CD antibody AQP2 antibody AQP2_HUMAN antibody AQPCD antibody Aquaporin 2 collecting duct antibody Aquaporin CD antibody Aquaporin-2 antibody Aquaporin-CD antibody Aquaporin2 antibody Aquaporine 2 antibody Collecting duct water channel protein antibody MGC34501 antibody Water channel aquaporin 2 antibody Water channel protein for renal collecting duct antibody WCH CD antibody WCH-CD antibody WCHCD antibody |
| Product description | AQP2 is found in the apical cell membranes of the kidney's collecting duct principal cells and in intracellular vesicles located throughout the cell. It is the only aquaporin regulated by vasopressin.[5] The basic job of aquaporin 2 is to reabsorb water from the urine while its being removed from the blood by the kidney. Aquaporin 2 is in kidney epithelial cells and usually lies dormant in intracellular vesicle membranes. When it is needed, vasopressin binds to the cell surface vasopressin receptor thereby activating a signaling pathway that causes the aquaporin 2 containing vesicles to fuse with the plasma membrane, so the aquaporin 2 can be used by the cell. Mutations in this channel are associated with nephrogenic diabetes insipidus, which can be autosomal dominant or recessive. Mutations in the vasopressin receptor cause a similar X-linked phenotype. Lithium, which is often used to treat bipolar disorder, can cause acquired diabetes insipidus (characterized by the excretion of large volumes of dilute urine) by decreasing the expression of the AQP2 gene. The expression of the AQP2 gene is increased during conditions associated with water retention such as pregnancy and congestive heart failure. |
| Immunogen | Purified recombinant fragment of human AQP2 (AA: 149-271) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500-1:2,000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | ADH water channel antibody AQP 2 antibody AQP CD antibody AQP-2 antibody AQP-CD antibody AQP2 antibody AQP2_HUMAN antibody AQPCD antibody Aquaporin 2 collecting duct antibody Aquaporin CD antibody Aquaporin-2 antibody Aquaporin-CD antibody Aquaporin2 antibody Aquaporine 2 antibody Collecting duct water channel protein antibody MGC34501 antibody Water channel aquaporin 2 antibody Water channel protein for renal collecting duct antibody WCH CD antibody WCH-CD antibody WCHCD antibody |
|---|---|
| Molecular Weight(MW) | 28.8kDa |
| Cellular Localization | Apical cell membrane. Basolateral cell membrane. Cytoplasmic vesicle membrane. Golgi apparatus, trans-Golgi network membrane. Shuttles from vesicles to the apical membrane. Vasopressin-regulated phosphorylation is required for translocation to the apical cell membrane. PLEKHA8/FAPP2 is required to transport AQP2 from the TGN to sites where AQP2 is phosphorylated. |
| SwissProt ID | P41181 |
|---|
Application
Western blot analysis of AQP2 against human AQP2 (AA: 149-271) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-44, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of AQP2 against HEK293 (1) and AQP2 (AA: 149-271)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-44, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of AQP2 against K562 (1), HeLa (2), HCT116 (3), and SW480 (4) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-44, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.| Positive Control | K562, HeLa, HCT116, and SW480 cell |
|---|---|
| Application Notes | WB:1:500-1:2,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
