Application
Western blot analysis of CD100 against human CD100 (AA: extra 590-734) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-47, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of CD100 against HEK293 (1) and CD100 (AA: extra 590-734)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-47, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Immunohistochemical analysis of paraffin-embedded cervical cancer tissue using anti-CD100 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1710-47, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Application
Immunohistochemical analysis of paraffin-embedded bladder cancer tissue using anti-CD100 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1710-47, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Application
Flow cytometric analysis of CD100 was done on Ramos cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-47, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).Application
Flow cytometric analysis of CD100 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-47, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | CD100 [5H6E3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | A8 antibody BB 18 antibody BB18 antibody CD 100 antibody CD100 antibody CD100 antigen antibody Coll 4 antibody COLL4 antibody Collapsin 4 antibody Collapsin4 antibody GR3 antibody Leukocyte activation antigen CD100 antibody M sema G antibody MSEMA antibody SEM4D_HUMAN antibody Sema 4d antibody Sema domain immunoglobulin domain Ig transmembrane domain TM and short cytoplasmic domain semaphorin 4D antibody Sema H antibody SEMA J antibody Sema4d antibody Semacl 2 antibody Semacl2 antibody SemaH antibody SEMAJ antibody Semaphorin 4D antibody Semaphorin C like 2 antibody Semaphorin H antibody Semaphorin J antibody Semaphorin-4D antibody Semaphorin4D antibody SemaphorinJ antibody Semcl 2 antibody Semcl2 antibody |
| Product description | SEMA4D (Semaphorin 4D) is a Protein Coding gene. Diseases associated with SEMA4D include Hemorrhagic Fever With Renal Syndrome. Among its related pathways are Guidance Cues and Growth Cone Motility and Developmental Biology. GO annotations related to this gene include receptor binding and transmembrane signaling receptor activity. An important paralog of this gene is SEMA4B. |
| Immunogen | Purified recombinant fragment of human CD100 (AA: extra 590-734) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | A8 antibody BB 18 antibody BB18 antibody CD 100 antibody CD100 antibody CD100 antigen antibody Coll 4 antibody COLL4 antibody Collapsin 4 antibody Collapsin4 antibody GR3 antibody Leukocyte activation antigen CD100 antibody M sema G antibody MSEMA antibody SEM4D_HUMAN antibody Sema 4d antibody Sema domain immunoglobulin domain Ig transmembrane domain TM and short cytoplasmic domain semaphorin 4D antibody Sema H antibody SEMA J antibody Sema4d antibody Semacl 2 antibody Semacl2 antibody SemaH antibody SEMAJ antibody Semaphorin 4D antibody Semaphorin C like 2 antibody Semaphorin H antibody Semaphorin J antibody Semaphorin-4D antibody Semaphorin4D antibody SemaphorinJ antibody Semcl 2 antibody Semcl2 antibody |
|---|---|
| Molecular Weight(MW) | 96.2kDa |
| Cellular Localization | Membrane. |
| SwissProt ID | Q92854 |
|---|
Application
Western blot analysis of CD100 against human CD100 (AA: extra 590-734) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-47, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of CD100 against HEK293 (1) and CD100 (AA: extra 590-734)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-47, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Immunohistochemical analysis of paraffin-embedded cervical cancer tissue using anti-CD100 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1710-47, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Immunohistochemical analysis of paraffin-embedded bladder cancer tissue using anti-CD100 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1710-47, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Flow cytometric analysis of CD100 was done on Ramos cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-47, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
Application
Flow cytometric analysis of CD100 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-47, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | K562 cells 、Ramos cells 、cervical cancer tissues 、bladder cancer tissues |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
