Application
Western blot analysis of GRIN2B against human GRIN2B (AA: extra 27-163) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-53, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of CD193 against HEK293 (1) and CD193 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-53, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of CD193 was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-53, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).Application
Flow cytometric analysis of CD193 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-53, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | CD193 [8E9H10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | B chemokine receptor antibody b-chemokine receptor antibody C C chemokine receptor type 3 antibody C C CKR 3 antibody C-C chemokine receptor type 3 antibody C-C CKR-3 antibody CC chemokine receptor 3 antibody CC chemokine receptor type 3 antibody CC CKR 3 antibody CC R3 antibody CC-CKR-3 antibody CCCKR3 antibody CCR 3 antibody CCR-3 antibody CCR3 antibody CCR3_HUMAN antibody CD 193 antibody CD193 antibody CD193 antigen antibody Chemokine (C C motif) receptor 3 antibody chemokine (C-C motif) receptor 3 antibody Chemokine (CC) receptor 1 like 2 antibody Chemokine CC motif receptor 3 antibody Chemokine receptor 3 antibody CK R3 antibody CKR 3 antibody CKR3 antibody CMKB R3 antibody CMKBR 3 antibody CMKBR1L2 antibody CMKBR3 antibody Eosinophil CC chemokine receptor 3 antibody Eosinophil eotaxin receptor antibody Eotaxin receptor antibody Macrophage inflammatory protein 1 alpha receptor like 2 antibody MGC102841 antibody MIP1 Alpha RL2 antibody |
| Product description | The protein encoded by this gene is a receptor for C-C type chemokines. It belongs to family 1 of the G protein-coupled receptors. This receptor binds and responds to a variety of chemokines, including eotaxin (CCL11), eotaxin-3 (CCL26), MCP-3 (CCL7), MCP-4 (CCL13), and RANTES (CCL5). It is highly expressed in eosinophils and basophils, and is also detected in TH1 and TH2 cells, as well as in airway epithelial cells. This receptor may contribute to the accumulation and activation of eosinophils and other inflammatory cells in the allergic airway. It is also known to be an entry co-receptor for HIV-1. This gene and seven other chemokine receptor genes form a chemokine receptor gene cluster on the chromosomal region 3p21. Alternatively spliced transcript variants have been described. |
| Immunogen | Purified recombinant fragment of human CD193 (AA: extra mix) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | B chemokine receptor antibody b-chemokine receptor antibody C C chemokine receptor type 3 antibody C C CKR 3 antibody C-C chemokine receptor type 3 antibody C-C CKR-3 antibody CC chemokine receptor 3 antibody CC chemokine receptor type 3 antibody CC CKR 3 antibody CC R3 antibody CC-CKR-3 antibody CCCKR3 antibody CCR 3 antibody CCR-3 antibody CCR3 antibody CCR3_HUMAN antibody CD 193 antibody CD193 antibody CD193 antigen antibody Chemokine (C C motif) receptor 3 antibody chemokine (C-C motif) receptor 3 antibody Chemokine (CC) receptor 1 like 2 antibody Chemokine CC motif receptor 3 antibody Chemokine receptor 3 antibody CK R3 antibody CKR 3 antibody CKR3 antibody CMKB R3 antibody CMKBR 3 antibody CMKBR1L2 antibody CMKBR3 antibody Eosinophil CC chemokine receptor 3 antibody Eosinophil eotaxin receptor antibody Eotaxin receptor antibody Macrophage inflammatory protein 1 alpha receptor like 2 antibody MGC102841 antibody MIP1 Alpha RL2 antibody |
|---|---|
| Molecular Weight(MW) | 41kDa |
| Cellular Localization | Cell membrane. |
| SwissProt ID | P51677 |
|---|
Application
Western blot analysis of GRIN2B against human GRIN2B (AA: extra 27-163) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-53, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of CD193 against HEK293 (1) and CD193 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-53, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of CD193 was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-53, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
Application
Flow cytometric analysis of CD193 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-53, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | HL-60 cells, K562 cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
