Application
Western blot analysis of CD207 against human CD207 (AA: extra 155-328) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-55, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of CD207 against HEK293 (1) and CD207 (AA: extra 155-328)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-55, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of CD207 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-55, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | CD207 [1C5A1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C type lectin domain family 4 member K antibody C-type lectin domain family 4 member K antibody CD 207 antibody CD207 antibody CD207 antigen antibody CD207 antigen, langerin antibody CD207 molecule antibody CLC4K_HUMAN antibody CLEC 4K antibody CLEC4K antibody Langerhans cell specific c type lectin antibody Langerin antibody RGD1565913 antibody |
| Product description | The protein encoded by this gene is expressed only in Langerhans cells which are immature dendritic cells of the epidermis and mucosa. It is localized in the Birbeck granules, organelles present in the cytoplasm of Langerhans cells and consisting of superimposed and zippered membranes. It is a C-type lectin with mannose binding specificity, and it has been proposed that mannose binding by this protein leads to internalization of antigen into Birbeck granules and providing access to a nonclassical antigen-processing pathway. Mutations in this gene result in Birbeck granules deficiency or loss of sugar binding activity. |
| Immunogen | Purified recombinant fragment of human CD207 (AA: extra 155-328) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | C type lectin domain family 4 member K antibody
C-type lectin domain family 4 member K antibody
CD 207 antibody
CD207 antibody
CD207 antigen antibody
CD207 antigen langerin antibody CD207 molecule antibody CLC4K_HUMAN antibody CLEC 4K antibody CLEC4K antibody Langerhans cell specific c type lectin antibody Langerin antibody RGD1565913 antibody |
|---|---|
| Molecular Weight(MW) | 36.7kDa |
| Cellular Localization | Membrane. Found in Birbeck granules (BGs), which are organelles consisting of superimposed and zippered membranes. |
| SwissProt ID | Q9UJ71 |
|---|
Application
Western blot analysis of CD207 against human CD207 (AA: extra 155-328) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-55, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of CD207 against HEK293 (1) and CD207 (AA: extra 155-328)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-55, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of CD207 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-55, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | K562 cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
