Application
Western blot analysis of P2RY12 against human P2RY12 (AA: extra mix) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-57, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of P2RY12 against HEK293 (1) and P2RY12 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-57, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of P2RY12 against PC-3 (1) and C6 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-57, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of P2RY12 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-57, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | P2RY12 [1C2A9] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ADP glucose receptor antibody ADP-glucose receptor antibody ADPG R antibody ADPG-R antibody ADPGR antibody BDPLT8 antibody G protein coupled receptor SP1999 antibody Gi coupled ADP receptor HORK 3 antibody Gi coupled ADP receptor HORK3 antibody HORK 3 antibody HORK3 antibody P2RY 12 antibody P2RY12 antibody P2T(AC) antibody P2Y 12 antibody P2Y purinoceptor 12 antibody P2Y(12)R antibody P2Y(AC) antibody P2Y(ADP) antibody P2Y(cyc) antibody P2Y12 antibody P2Y12 platelet ADP receptor antibody P2Y12_HUMAN antibody Platelet ADP receptor antibody Purinergic receptor P2RY12 antibody Purinergic receptor P2Y G protein coupled 12 antibody Purinergic receptor P2Y12 antibody Putative G protein coupled receptor antibody SP 1999 antibody SP1999 antibody |
| Product description | The product of this gene belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor is involved in platelet aggregation, and is a potential target for the treatment of thromboembolisms and other clotting disorders. Mutations in this gene are implicated in bleeding disorder, platelet type 8 (BDPLT8). Alternative splicing results in multiple transcript variants of this gene. |
| Immunogen | Purified recombinant fragment of human P2RY12 (AA: extra mix) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | ADP glucose receptor antibody ADP-glucose receptor antibody ADPG R antibody ADPG-R antibody ADPGR antibody BDPLT8 antibody G protein coupled receptor SP1999 antibody Gi coupled ADP receptor HORK 3 antibody Gi coupled ADP receptor HORK3 antibody HORK 3 antibody HORK3 antibody P2RY 12 antibody P2RY12 antibody P2T(AC) antibody P2Y 12 antibody P2Y purinoceptor 12 antibody P2Y(12)R antibody P2Y(AC) antibody P2Y(ADP) antibody P2Y(cyc) antibody P2Y12 antibody P2Y12 platelet ADP receptor antibody P2Y12_HUMAN antibody Platelet ADP receptor antibody Purinergic receptor P2RY12 antibody Purinergic receptor P2Y G protein coupled 12 antibody Purinergic receptor P2Y12 antibody Putative G protein coupled receptor antibody SP 1999 antibody SP1999 antibody |
|---|---|
| Molecular Weight(MW) | 39.4kDa |
| Cellular Localization | Cell membrane. |
| SwissProt ID | Q9H244 |
|---|
Application
Western blot analysis of P2RY12 against human P2RY12 (AA: extra mix) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-57, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of P2RY12 against HEK293 (1) and P2RY12 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-57, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of P2RY12 against PC-3 (1) and C6 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-57, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of P2RY12 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-57, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | PC-3 and C6 cell lysate, Hela cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
