Application
Western blot analysis of BTLA against human BTLA (AA: extra 31-157) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-61, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of BTLA against HEK293 (1) and BTLA (AA: extra 31-157)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-61, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Immunocytochemistry staining of BTLA in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1710-61, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor| Product Name | BTLA [7B8B4] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | B and T lymphocyte associated protein antibody B and T lymphocyte attenuator antibody B and T lymphocyte associated antibody B- and T-lymphocyte attenuator antibody B- and T-lymphocyte-associated protein antibody BTLA antibody BTLA_HUMAN antibody BTLA1 antibody CD272 antibody CD272 antigen antibody FLJ16065 antibody MGC129743 antibody |
| Product description | This gene encodes a member of the immunoglobulin superfamily. The encoded protein contains a single immunoglobulin (Ig) domain and is a receptor that relays inhibitory signals to suppress the immune response. Alternative splicing results in multiple transcript variants. Polymorphisms in this gene have been associated with an increased risk of rheumatoid arthritis. |
| Immunogen | Purified recombinant fragment of human BTLA (AA: extra 31-157) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICC |
| WB:1:500-1:2,000 ICC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | B and T lymphocyte associated protein antibody B and T lymphocyte attenuator antibody B and T lymphocyte associated antibody B- and T-lymphocyte attenuator antibody B- and T-lymphocyte-associated protein antibody BTLA antibody BTLA_HUMAN antibody BTLA1 antibody CD272 antibody CD272 antigen antibody FLJ16065 antibody MGC129743 antibody |
|---|---|
| Molecular Weight(MW) | 32.8kd |
| Cellular Localization | Membrane. |
| SwissProt ID | Q7Z6A9 |
|---|
Application
Western blot analysis of BTLA against human BTLA (AA: extra 31-157) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-61, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of BTLA against HEK293 (1) and BTLA (AA: extra 31-157)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-61, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Immunocytochemistry staining of BTLA in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1710-61, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor| Positive Control | Hela cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
