Application
Western blot analysis of VISTA against human VISTA (AA: extra 33-194) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-63, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of VISTA was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-63, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | VISTA [6E4H3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | B7 H5 antibody B7H5 antibody C10orf54 antibody chromosome 10 open reading frame 54 antibody DD1alpha antibody GI24 antibody GI24_HUMAN antibody PD-1H antibody PDCD1 homolog antibody Platelet receptor Gi24 antibody PP2135 antibody sisp 1 antibody SISP1 antibody stress induced secreted protein 1 antibody UNQ730/PRO1412 antibody V domain Ig suppressor of T cell activation antibody V set domain containing immunoregulatory receptor antibody V set immunoregulatory receptor antibody V type immunoglobulin domain containing suppressor of T cell activation antibody VISTA antibody |
| Product description | VSIR (V-Set Immunoregulatory Receptor) is a Protein Coding gene. Diseases associated with VSIR include Parasagittal Meningioma and Solar Retinopathy. Among its related pathways are T Cell Co-Signaling Pathway: Ligand-Receptor Interactions and NF-kappaB Signaling. |
| Immunogen | Purified recombinant fragment of human VISTA (AA: extra 33-194) expressed in HEK293 cells. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | B7 H5 antibody B7H5 antibody C10orf54 antibody chromosome 10 open reading frame 54 antibody DD1alpha antibody GI24 antibody GI24_HUMAN antibody PD-1H antibody PDCD1 homolog antibody Platelet receptor Gi24 antibody PP2135 antibody sisp 1 antibody SISP1 antibody stress induced secreted protein 1 antibody UNQ730/PRO1412 antibody V domain Ig suppressor of T cell activation antibody V set domain containing immunoregulatory receptor antibody V set immunoregulatory receptor antibody V type immunoglobulin domain containing suppressor of T cell activation antibody VISTA antibody |
|---|---|
| Molecular Weight(MW) | 34kd |
| Cellular Localization | Membrane. |
| SwissProt ID | Q9H7M9 |
|---|
Application
Western blot analysis of VISTA against human VISTA (AA: extra 33-194) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-63, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of VISTA was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-63, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | Jurkat cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
