Application
Western blot analysis of CD119 against human CD119 (AA: extra 18-245) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-66, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of CD119 against HEK293 (1) and CD119 (AA: extra 18-245)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-66, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of CD119 against HepG2 (1) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-66, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of CD119 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-66, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | CD119 [3E1A9] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Antiviral Protein Type II antibody Antiviral protein, type 2 antibody AVP type II antibody AVP, type 2 antibody CD 119 antibody CD119 antibody CD119 antigen antibody CDw119 antibody FLJ45734 antibody IFN gamma R alpha antibody IFN gamma R antibody IFN gamma R1 antibody IFN-gamma receptor 1 antibody IFN-gamma-R1 antibody IFNG R1 antibody IFNGR 1 antibody IFNGR antibody IFNGR1 antibody Immune interferon receptor 1 antibody Immune interferon receptor for antibody INGR1_HUMAN antibody Interferon gamma receptor 1 antibody Interferon gamma receptor alpha chain antibody Interferon gamma receptor alpha chain precursor antibody |
| Product description | This gene (IFNGR1) encodes the ligand-binding chain (alpha) of the gamma interferon receptor. Human interferon-gamma receptor is a heterodimer of IFNGR1 and IFNGR2. A genetic variation in IFNGR1 is associated with susceptibility to Helicobacter pylori infection. In addition, defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease, also known as familial disseminated atypical mycobacterial infection. |
| Immunogen | Purified recombinant fragment of human CD119 (AA: extra 18-245) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | Antiviral Protein Type II antibody
Antiviral protein type 2 antibody AVP type II antibody AVP type 2 antibody CD 119 antibody CD119 antibody CD119 antigen antibody CDw119 antibody FLJ45734 antibody IFN gamma R alpha antibody IFN gamma R antibody IFN gamma R1 antibody IFN-gamma receptor 1 antibody IFN-gamma-R1 antibody IFNG R1 antibody IFNGR 1 antibody IFNGR antibody IFNGR1 antibody Immune interferon receptor 1 antibody Immune interferon receptor for antibody INGR1_HUMAN antibody Interferon gamma receptor 1 antibody Interferon gamma receptor alpha chain antibody Interferon gamma receptor alpha chain precursor antibody |
|---|---|
| Molecular Weight(MW) | 54.4kDa |
| Cellular Localization | Membrane. |
| SwissProt ID | P15260 |
|---|
Application
Western blot analysis of CD119 against human CD119 (AA: extra 18-245) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-66, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of CD119 against HEK293 (1) and CD119 (AA: extra 18-245)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-66, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of CD119 against HepG2 (1) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-66, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of CD119 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-66, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | HepG2 cell lysate, K562 cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
