Application
Western blot analysis of CD16 against human CD16 (AA: extra 17-208) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-67, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of CD16 against HEK293 (1) and CD16 (AA: extra 17-208)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-67, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of CD16 was done on Ramos cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-67, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | CD16 [2G10A9] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD 16 antibody CD 16a antibody CD16 antibody CD16a antibody CD16a antigen antibody CD16B antibody CD16b antigen antibody Fc fragment of IgG antibody Fc fragment of IgG low affinity IIIa receptor (CD16) antibody Fc fragment of IgG low affinity IIIa receptor antibody Fc fragment of IgG receptor IIIa antibody Fc fragment of IgG, low affinity III, receptor (CD16) antibody Fc fragment of IgG, low affinity III, receptor for (CD16) antibody Fc fragment of IgG, low affinity IIIa, receptor (CD16) antibody Fc fragment of IgG, low affinity IIIa, receptor (CD16a) antibody Fc fragment of IgG, low affinity IIIa, receptor for antibody Fc fragment of IgG, low affinity IIIb, receptor (CD16b) antibody Fc fragment of IgG, low affinity IIIb, receptor for (CD16) antibody Fc gamma R3 antibody Fc gamma receptor III 2 (CD 16) antibody Fc gamma receptor III A antibody Fc gamma receptor IIIA antibody Fc gamma receptor IIIb (CD 16) antibody Fc gamma RIII alpha antibody Fc gamma RIII antibody Fc gamma RIII beta antibody Fc gamma RIIIa antibody Fc gamma RIIIb antibody Fc of IgG antibody Fc-gamma receptor III2 (CD 16) antibody Fc-gamma receptor III2 (CD16) antibody Fc-gamma receptor IIIb (CD16) antibody Fc-gamma RIII antibody Fc-gamma RIII-alpha antibody Fc-gamma RIIIa antibody FCG 3 antibody FCG3 antibody FCG3A_HUMAN antibody FCgammaRIIIA antibody FCGR 3 antibody FCGR 3A antibody FCGR3 antibody FCGR3A antibody FCGR3A protein antibody FCGRIII antibody FCGRIII-2 antibody FcR 10 antibody FcR-10 antibody FcR10 antibody FcRIII antibody FcRIIIa antibody IGFR 3 antibody IGFR3 antibody IgG Fc receptor III 1 antibody IgG Fc receptor III 2 antibody IgG Fc receptor III-2 antibody IMD20 antibody immunoglobulin G Fc receptor III antibody immunoglobulin G Fc receptor III-2 antibody Low affinity IIIa receptor antibody Low affinity immunoglobulin gamma Fc region receptor III A antibody Low affinity immunoglobulin gamma Fc region receptor III-A antibody Low affinity immunoglobulin gamma Fc region receptor IIIB antibody neutrophil-specific antigen NA antibody |
| Product description | This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other other antibody-dependent responses. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Immunogen | Purified recombinant fragment of human CD16 (AA: extra 17-208) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | CD 16 antibody
CD 16a antibody
CD16 antibody
CD16a antibody
CD16a antigen antibody
CD16B antibody
CD16b antigen antibody
Fc fragment of IgG antibody
Fc fragment of IgG low affinity IIIa receptor (CD16) antibody
Fc fragment of IgG low affinity IIIa receptor antibody
Fc fragment of IgG receptor IIIa antibody
Fc fragment of IgG low affinity III receptor (CD16) antibody Fc fragment of IgG low affinity III receptor for (CD16) antibody Fc fragment of IgG low affinity IIIa receptor (CD16) antibody Fc fragment of IgG low affinity IIIa receptor (CD16a) antibody Fc fragment of IgG low affinity IIIa receptor for antibody Fc fragment of IgG low affinity IIIb receptor (CD16b) antibody Fc fragment of IgG low affinity IIIb receptor for (CD16) antibody Fc gamma R3 antibody Fc gamma receptor III 2 (CD 16) antibody Fc gamma receptor III A antibody Fc gamma receptor IIIA antibody Fc gamma receptor IIIb (CD 16) antibody Fc gamma RIII alpha antibody Fc gamma RIII antibody Fc gamma RIII beta antibody Fc gamma RIIIa antibody Fc gamma RIIIb antibody Fc of IgG antibody Fc-gamma receptor III2 (CD 16) antibody Fc-gamma receptor III2 (CD16) antibody Fc-gamma receptor IIIb (CD16) antibody Fc-gamma RIII antibody Fc-gamma RIII-alpha antibody Fc-gamma RIIIa antibody FCG 3 antibody FCG3 antibody FCG3A_HUMAN antibody FCgammaRIIIA antibody FCGR 3 antibody FCGR 3A antibody FCGR3 antibody FCGR3A antibody FCGR3A protein antibody FCGRIII antibody FCGRIII-2 antibody FcR 10 antibody FcR-10 antibody FcR10 antibody FcRIII antibody FcRIIIa antibody IGFR 3 antibody IGFR3 antibody IgG Fc receptor III 1 antibody IgG Fc receptor III 2 antibody IgG Fc receptor III-2 antibody IMD20 antibody immunoglobulin G Fc receptor III antibody immunoglobulin G Fc receptor III-2 antibody Low affinity IIIa receptor antibody Low affinity immunoglobulin gamma Fc region receptor III A antibody Low affinity immunoglobulin gamma Fc region receptor III-A antibody Low affinity immunoglobulin gamma Fc region receptor IIIB antibody neutrophil-specific antigen NA antibody |
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| Molecular Weight(MW) | 29kDa |
| Cellular Localization | Cell membrane. Secreted. Exists also as a soluble receptor. |
| SwissProt ID | P08637 |
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Application
Western blot analysis of CD16 against human CD16 (AA: extra 17-208) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-67, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of CD16 against HEK293 (1) and CD16 (AA: extra 17-208)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-67, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of CD16 was done on Ramos cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-67, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | Ramos cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
