Application
Western blot analysis of CD122 against human CD122 (AA: extra 27-240) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-70, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of CD122 against HEK293 (1) and CD122 (AA: extra 27-240)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-70, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of CD122 was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-70, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).Application
Flow cytometric analysis of CD122 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-70, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | CD122 [1G11C6] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD122 antibody CD122 antigen antibody High affinity IL 2 receptor beta subunit antibody High affinity IL 2 receptor subunit beta antibody High affinity IL-2 receptor subunit beta antibody IL-2 receptor subunit beta antibody IL-2R subunit beta antibody IL-2RB antibody IL15RB antibody IL2 receptor antibody IL2RB antibody IL2RB_HUMAN antibody Interleukin 2 receptor beta antibody Interleukin-2 receptor subunit beta antibody P70 75 antibody P70-75 antibody P7075 antibody p75 antibody |
| Product description | The interleukin 2 receptor, which is involved in T cell-mediated immune responses, is present in 3 forms with respect to ability to bind interleukin 2. The low affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high affinity forms of the receptor are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. The protein encoded by this gene represents the beta subunit and is a type I membrane protein. The use of alternative promoters results in multiple transcript variants encoding the same protein. The protein is primarily expressed in the hematopoietic system. The use by some variants of an alternate promoter in an upstream long terminal repeat (LTR) results in placenta-specific expression. |
| Immunogen | Purified recombinant fragment of human CD122 (AA: extra 27-240) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | CD122 antibody CD122 antigen antibody High affinity IL 2 receptor beta subunit antibody High affinity IL 2 receptor subunit beta antibody High affinity IL-2 receptor subunit beta antibody IL-2 receptor subunit beta antibody IL-2R subunit beta antibody IL-2RB antibody IL15RB antibody IL2 receptor antibody IL2RB antibody IL2RB_HUMAN antibody Interleukin 2 receptor beta antibody Interleukin-2 receptor subunit beta antibody P70 75 antibody P70-75 antibody P7075 antibody p75 antibody |
|---|---|
| Molecular Weight(MW) | 61kDa |
| Cellular Localization | Membrane. |
| SwissProt ID | P14784 |
|---|
Application
Western blot analysis of CD122 against human CD122 (AA: extra 27-240) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-70, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of CD122 against HEK293 (1) and CD122 (AA: extra 27-240)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-70, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of CD122 was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-70, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
Application
Flow cytometric analysis of CD122 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-70, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | HL-60 cells, Jurkat cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
