Application
Western blot analysis of CD191 against human CD191 (AA: extra mix) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-71, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of CD191 against HEK293 (1) and CD191 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-71, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of CD191 against HepG2 (1) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-71, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of CD191 was done on Ramos cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-71, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | CD191 [8E10F9] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C C chemokine receptor type 1 antibody C C CKR 1 antibody C-C chemokine receptor type 1 antibody C-C CKR-1 antibody CC CKR 1 antibody CC-CKR-1 antibody CCR 1 antibody CCR-1 antibody CCR1 antibody CCR1_HUMAN antibody CD 191 antibody CD191 antibody CD191 antigen antibody Chemokine (C C motif) receptor 1 antibody Chemokine C C motif receptor 1 antibody CKR 1 antibody CKR1 antibody CMKBR 1 antibody CMKBR1 antibody CMKR 1 antibody CMKR1 antibody HM145 antibody LD78 receptor antibody Macrophage inflammatory protein 1 alpha /Rantes receptor antibody Macrophage inflammatory protein 1 alpha receptor antibody Macrophage inflammatory protein 1-alpha receptor antibody Mip 1a R antibody MIP 1alpha R antibody MIP-1alpha-R antibody MIP1aR antibody RANTES R antibody RANTES receptor antibody RANTES-R antibody SCYAR1 antibody |
| Product description | This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. The ligands of this receptor include macrophage inflammatory protein 1 alpha (MIP-1 alpha), regulated on activation normal T expressed and secreted protein (RANTES), monocyte chemoattractant protein 3 (MCP-3), and myeloid progenitor inhibitory factor-1 (MPIF-1). Chemokines and their receptors mediated signal transduction are critical for the recruitment of effector immune cells to the site of inflammation. Knockout studies of the mouse homolog suggested the roles of this gene in host protection from inflammatory response, and susceptibility to virus and parasite. This gene and other chemokine receptor genes, including CCR2, CCRL2, CCR3, CCR5 and CCXCR1, are found to form a gene cluster on chromosome 3p. |
| Immunogen | Purified recombinant fragment of human CD191 (AA: extra mix) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | C C chemokine receptor type 1 antibody C C CKR 1 antibody C-C chemokine receptor type 1 antibody C-C CKR-1 antibody CC CKR 1 antibody CC-CKR-1 antibody CCR 1 antibody CCR-1 antibody CCR1 antibody CCR1_HUMAN antibody CD 191 antibody CD191 antibody CD191 antigen antibody Chemokine (C C motif) receptor 1 antibody Chemokine C C motif receptor 1 antibody CKR 1 antibody CKR1 antibody CMKBR 1 antibody CMKBR1 antibody CMKR 1 antibody CMKR1 antibody HM145 antibody LD78 receptor antibody Macrophage inflammatory protein 1 alpha /Rantes receptor antibody Macrophage inflammatory protein 1 alpha receptor antibody Macrophage inflammatory protein 1-alpha receptor antibody Mip 1a R antibody MIP 1alpha R antibody MIP-1alpha-R antibody MIP1aR antibody RANTES R antibody RANTES receptor antibody RANTES-R antibody SCYAR1 antibody |
|---|---|
| Molecular Weight(MW) | 41.2kDa |
| Cellular Localization | Cell membrane. |
| SwissProt ID | P32246 |
|---|
Application
Western blot analysis of CD191 against human CD191 (AA: extra mix) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-71, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of CD191 against HEK293 (1) and CD191 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-71, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of CD191 against HepG2 (1) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-71, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of CD191 was done on Ramos cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-71, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | HepG2 cell lysate, Ramos cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
