Application
Western blot analysis of CD161 against human CD161 (AA: extra 67-225) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-77, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of CD161 against HEK293 (1) and CD161 (AA: extra 67-225)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-77, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of CD161 was done on Raji cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-77, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | CD161 [4C6A11] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C-type lectin domain family 5 member B antibody CD161 antibody CLEC5B antibody HNKR-P1a antibody Killer Cell Lectin like Receptor Subfamily B Member 1 antibody Killer cell lectin-like receptor subfamily B member 1 antibody KLRB1 antibody KLRB1_HUMAN antibody Natural killer cell surface protein P1A antibody NKR antibody NKR P1 antibody NKR-P1A antibody NKRP1 antibody NKRP1A antibody |
| Product description | Natural killer (NK) cells are lymphocytes that mediate cytotoxicity and secrete cytokines after immune stimulation. Several genes of the C-type lectin superfamily, including the rodent NKRP1 family of glycoproteins, are expressed by NK cells and may be involved in the regulation of NK cell function. The KLRB1 protein contains an extracellular domain with several motifs characteristic of C-type lectins, a transmembrane domain, and a cytoplasmic domain. The KLRB1 protein is classified as a type II membrane protein because it has an external C terminus. |
| Immunogen | Purified recombinant fragment of human CD161 (AA: extra 67-225) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | C-type lectin domain family 5 member B antibody CD161 antibody CLEC5B antibody HNKR-P1a antibody Killer Cell Lectin like Receptor Subfamily B Member 1 antibody Killer cell lectin-like receptor subfamily B member 1 antibody KLRB1 antibody KLRB1_HUMAN antibody Natural killer cell surface protein P1A antibody NKR antibody NKR P1 antibody NKR-P1A antibody NKRP1 antibody NKRP1A antibody |
|---|---|
| Molecular Weight(MW) | 25.4kDa |
| Cellular Localization | Membrane. |
| SwissProt ID | Q12918 |
|---|
Application
Western blot analysis of CD161 against human CD161 (AA: extra 67-225) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-77, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of CD161 against HEK293 (1) and CD161 (AA: extra 67-225)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-77, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of CD161 was done on Raji cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-77, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | Raji cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
