Application
Western blot analysis of CD305 against CD305 (AA: extra 22-165) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-81, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of EM1710-81 against HEK293 (1) and CD305 (AA: extra 22-165)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-81, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of CD305 was done on Raji cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-81, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | CD305 [1G8B6] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 5133400O11Rik antibody CD305 antibody D7Bwg0421e antibody hLAIR1 antibody LAIR 1 antibody LAIR-1 antibody LAIR1 antibody LAIR1 protein antibody LAIR1_HUMAN antibody Leukocyte associated Ig like receptor 1 antibody Leukocyte associated immunoglobulin like receptor 1 antibody Leukocyte-associated immunoglobulin-like receptor 1 antibody |
| Product description | The protein encoded by this gene is an inhibitory receptor found on peripheral mononuclear cells, including natural killer cells, T cells, and B cells. Inhibitory receptors regulate the immune response to prevent lysis of cells recognized as self. The gene is a member of both the immunoglobulin superfamily and the leukocyte-associated inhibitory receptor family. The gene maps to a region of 19q13.4 called the leukocyte receptor cluster, which contains at least 29 genes encoding leukocyte-expressed receptors of the immunoglobulin superfamily. The encoded protein has been identified as an anchor for tyrosine phosphatase SHP-1, and may induce cell death in myeloid leukemias. Alternative splicing results in multiple transcript variants. |
| Immunogen | Purified recombinant fragment of human CD305 (AA: extra 22-165) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | 5133400O11Rik antibody CD305 antibody D7Bwg0421e antibody hLAIR1 antibody LAIR 1 antibody LAIR-1 antibody LAIR1 antibody LAIR1 protein antibody LAIR1_HUMAN antibody Leukocyte associated Ig like receptor 1 antibody Leukocyte associated immunoglobulin like receptor 1 antibody Leukocyte-associated immunoglobulin-like receptor 1 antibody |
|---|---|
| Molecular Weight(MW) | 31.4kDa |
| Cellular Localization | Cell membrane. |
| SwissProt ID | Q6GTX8 |
|---|
Application
Western blot analysis of CD305 against CD305 (AA: extra 22-165) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-81, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1710-81 against HEK293 (1) and CD305 (AA: extra 22-165)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-81, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of CD305 was done on Raji cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-81, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | Raji cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
