Application
Western blot analysis of CD84 against human CD84 (AA: extra 22-225) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-83, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of EM1710-83 against HEK293 (1) and CD84 (AA: extra 22-225)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-83, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of CD84 was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-83, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | CD84 [5D12H10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD_antigen=CD84 antibody CD84 antibody CD84 antigen antibody CD84 molecule antibody CDw84 antibody Cell surface antigen MAX.3 antibody hCD84 antibody Hly9 beta antibody Hly9-beta antibody Leucocyte differentiation antigen CD84 antibody Leukocyte Antigen antibody Leukocyte antigen CD84 antibody Leukocyte differentiation antigen antibody Leukocyte differentiation antigen CD84 antibody LY9B antibody mCD84 antibody Signaling lymphocytic activation molecule 5 antibody SLAF5_HUMAN antibody SLAM family member 5 antibody SLAMF5 antibody |
| Product description | This gene encodes a membrane glycoprotein that is a member of the signaling lymphocyte activation molecule (SLAM) family. This family forms a subset of the larger CD2 cell-surface receptor Ig superfamily. The encoded protein is a homophilic adhesion molecule that is expressed in numerous immune cells types and is involved in regulating receptor-mediated signaling in those cells. Alternate splicing results in multiple transcript variants. |
| Immunogen | Purified recombinant fragment of human CD84 (AA: extra 22-225) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | CD_antigen=CD84 antibody CD84 antibody CD84 antigen antibody CD84 molecule antibody CDw84 antibody Cell surface antigen MAX.3 antibody hCD84 antibody Hly9 beta antibody Hly9-beta antibody Leucocyte differentiation antigen CD84 antibody Leukocyte Antigen antibody Leukocyte antigen CD84 antibody Leukocyte differentiation antigen antibody Leukocyte differentiation antigen CD84 antibody LY9B antibody mCD84 antibody Signaling lymphocytic activation molecule 5 antibody SLAF5_HUMAN antibody SLAM family member 5 antibody SLAMF5 antibody |
|---|---|
| Molecular Weight(MW) | 38.8kDa |
| Cellular Localization | Cell membrane. |
| SwissProt ID | Q9UIB8 |
|---|
Application
Western blot analysis of CD84 against human CD84 (AA: extra 22-225) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-83, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1710-83 against HEK293 (1) and CD84 (AA: extra 22-225)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-83, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of CD84 was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-83, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | HL-60 cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
