Application
Western blot analysis of CD226 against human CD226 (AA: extra 19-254) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-87, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of EM1710-87 against HEK293 (1) and CD226 (AA: extra 19-254)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-87, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.| Product Name | CD226 [8D11D5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | adhesion glycoprotein antibody CD226 antibody CD226 antigen antibody CD226 molecule antibody CD226_HUMAN antibody DNAM 1 antibody DNAM-1 antibody DNAM1 antibody DNAX accessory molecule 1 antibody platelet and T cell activation antigen 1 antibody PTA1 antibody T lineage specific activation antigen 1 antigen antibody TLiSA1 antibody |
| Product description | This gene encodes a glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. The protein mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. Alternative splicing results in multiple transcript variants. |
| Immunogen | Purified recombinant fragment of human CD226 (AA: extra 19-254) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500-1:2,000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | adhesion glycoprotein antibody CD226 antibody CD226 antigen antibody CD226 molecule antibody CD226_HUMAN antibody DNAM 1 antibody DNAM-1 antibody DNAM1 antibody DNAX accessory molecule 1 antibody platelet and T cell activation antigen 1 antibody PTA1 antibody T lineage specific activation antigen 1 antigen antibody TLiSA1 antibody |
|---|---|
| Molecular Weight(MW) | 38.6kDa |
| Cellular Localization | Membrane. |
| SwissProt ID | Q15762 |
|---|
Application
Western blot analysis of CD226 against human CD226 (AA: extra 19-254) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-87, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1710-87 against HEK293 (1) and CD226 (AA: extra 19-254)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-87, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.| Positive Control | Expressed by peripheral blood T-lymphocytes. |
|---|---|
| Application Notes | WB:1:500-1:2,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
