Application
Western blot analysis of TBC1D4 against human TBC1D4 (AA: 574-712) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-88, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of EM1710-88 against HEK293 (1) and TBC1D4 (AA: 574-712)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-88, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of TBC1D4 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-88, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | TBC1D4 [8A11A2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Acrg embryonic lethality (mouse) minimal region ortholog antibody Acrg embryonic lethality minimal region ortholog antibody Acrg embryonic lethality mouse minimal region ortholog antibody Akt substrate of 160 kDa antibody AS 160 antibody AS160 antibody BUB2 antibody CDC16 antibody KIAA0603 antibody NIDDM5 antibody TBC (Tre 2 BUB2 CDC16) domain containing protein antibody TBC Tre 2 BUB2 CDC16 domain containing protein antibody TBC1 D4 antibody TBC1 domain family member 4 antibody Tbc1d4 antibody TBCD4_HUMAN antibody Tre-2 antibody |
| Product description | This gene is a member of the Tre-2/BUB2/CDC16 domain family. The protein encoded by this gene is a Rab-GTPase-activating protein, and contains two phopshotyrosine-binding domains (PTB1 and PTB2), a calmodulin-binding domain (CBD), a Rab-GTPase domain, and multiple AKT phosphomotifs. This protein is thought to play an important role in glucose homeostasis by regulating the insulin-dependent trafficking of the glucose transporter 4 (GLUT4), important for removing glucose from the bloodstream into skeletal muscle and fat tissues. Reduced expression of this gene results in an increase in GLUT4 levels at the plasma membrane, suggesting that this protein is important in intracellular retention of GLUT4 under basal conditions. When exposed to insulin, this protein is phosphorylated, dissociates from GLUT4 vesicles, resulting in increased GLUT4 at the cell surface, and enhanced glucose transport. Phosphorylation of this protein by AKT is required for proper translocation of GLUT4 to the cell surface. Individuals homozygous for a mutation in this gene are at higher risk for type 2 diabetes and have higher levels of circulating glucose and insulin levels after glucose ingestion. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| Immunogen | Purified recombinant fragment of human TBC1D4 (AA: 574-712) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | Acrg embryonic lethality (mouse) minimal region ortholog antibody Acrg embryonic lethality minimal region ortholog antibody Acrg embryonic lethality mouse minimal region ortholog antibody Akt substrate of 160 kDa antibody AS 160 antibody AS160 antibody BUB2 antibody CDC16 antibody KIAA0603 antibody NIDDM5 antibody TBC (Tre 2 BUB2 CDC16) domain containing protein antibody TBC Tre 2 BUB2 CDC16 domain containing protein antibody TBC1 D4 antibody TBC1 domain family member 4 antibody Tbc1d4 antibody TBCD4_HUMAN antibody Tre-2 antibody |
|---|---|
| Molecular Weight(MW) | 146.5kDa |
| Cellular Localization | Cytoplasm. Isoform 2 shows a cytoplasmic perinuclear localization in a myoblastic cell line in resting and insulin-stimulated cells. |
| SwissProt ID | O60343 |
|---|
Application
Western blot analysis of TBC1D4 against human TBC1D4 (AA: 574-712) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-88, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1710-88 against HEK293 (1) and TBC1D4 (AA: 574-712)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-88, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of TBC1D4 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-88, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | Hela cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
