Application
Western blot analysis of P2RY2 against human P2RY2 (AA: extra mix) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-90, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of EM1710-90 against HEK293 (1) and P2RY2 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-90, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of P2RY2 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-90, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | P2RY2 [4F11E7] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATP receptor antibody HP2U antibody Human P2U nucleotide receptor mRNA complete cds antibody MGC20088 antibody MGC40010 antibody P2RU1 antibody P2ry2 antibody P2RY2_HUMAN antibody P2U antibody P2U nucleotide receptor antibody P2U purinoceptor 1 antibody P2U receptor 1 antibody P2U1 antibody P2UR antibody P2Y purinoceptor 2 antibody P2Y2 antibody P2Y2R antibody Purinergic receptor antibody Purinergic receptor P2Y G protein coupled 2 antibody Purinergic receptor P2Y2 antibody Purinoceptor P2Y2 antibody |
| Product description | The product of this gene belongs to the family of P2 receptors, which is activated by extracellular nucleotides and subdivided into P2X ligand-gated ion channels and P2Y G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor, found on many cell types, is activated by ATP and UTP and is reported to be overexpressed on some cancer cell types. It is involved in many cellular functions, such as proliferation, apoptosis and inflammation. Three transcript variants encoding the same protein have been identified for this gene. |
| Immunogen | Purified recombinant fragment of human P2RY2 (AA: extra mix) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | ATP receptor antibody HP2U antibody Human P2U nucleotide receptor mRNA complete cds antibody MGC20088 antibody MGC40010 antibody P2RU1 antibody P2ry2 antibody P2RY2_HUMAN antibody P2U antibody P2U nucleotide receptor antibody P2U purinoceptor 1 antibody P2U receptor 1 antibody P2U1 antibody P2UR antibody P2Y purinoceptor 2 antibody P2Y2 antibody P2Y2R antibody Purinergic receptor antibody Purinergic receptor P2Y G protein coupled 2 antibody Purinergic receptor P2Y2 antibody Purinoceptor P2Y2 antibody |
|---|---|
| Molecular Weight(MW) | 42.3kDa |
| Cellular Localization | Cell membrane. |
| SwissProt ID | P41231 |
|---|
Application
Western blot analysis of P2RY2 against human P2RY2 (AA: extra mix) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-90, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1710-90 against HEK293 (1) and P2RY2 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-90, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of P2RY2 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-90, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | Hela cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
