Application
Western blot analysis of CD203C against human CD203C (AA: extra 45-163) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-93, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of EM1710-93 against HEK293 (1) and CD203C (AA: extra 45-163)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-93, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Immunohistochemical analysis of paraffin-embedded human renal cancer tissue using anti-CD203C antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1710-93, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Application
Flow cytometric analysis of CD203C was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-93, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | CD203C [4C1H2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Alkaline phosphodiesterase I antibody ARHR2 antibody B10 antibody CD203c antibody CD203c antigen antibody dJ1005H11.3 (phosphodiesterase I/nucleotide pyrophosphatase 3) antibody dJ914N13.3 (phosphodiesterase I/nucleotide pyrophosphatase 3) antibody E NPP 3 antibody E-NPP 3 antibody Ectonucleotide pyrophosphatase/phosphodiesterase 3 antibody Ectonucleotide pyrophosphatase/phosphodiesterase family member 3 antibody ENPP3 antibody ENPP3_HUMAN antibody gp130RB13 6 antibody gp130RB136 antibody M6S1 antibody NPP1 antibody NPP3 antibody NPPase antibody NPPS antibody Nucleotide pyrophosphatase antibody PC 1 antibody PC-1 antibody PCA1 antibody PD Ibeta antibody PD-Ibeta antibody PDNP1 antibody PDNP3 antibody Phosphodiesterase I beta antibody Phosphodiesterase I/nucleotide pyrophosphatase 3 antibody phosphodiesterase i/nucleotide pyrophosphatase beta antibody |
| Product description | The protein encoded by this gene belongs to a series of ectoenzymes that are involved in hydrolysis of extracellular nucleotides. These ectoenzymes possess ATPase and ATP pyrophosphatase activities and are type II transmembrane proteins. Expression of the related rat mRNA has been found in a subset of immature glial cells and in the alimentary tract. The corresponding rat protein has been detected in the pancreas, small intestine, colon, and liver. The human mRNA is expressed in glioma cells, prostate, and uterus. Expression of the human protein has been detected in uterus, basophils, and mast cells. Two transcript variants, one protein coding and the other non-protein coding, have been found for this gene. |
| Immunogen | Purified recombinant fragment of human CD203C (AA: extra 45-163) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | Alkaline phosphodiesterase I antibody ARHR2 antibody B10 antibody CD203c antibody CD203c antigen antibody dJ1005H11.3 (phosphodiesterase I/nucleotide pyrophosphatase 3) antibody dJ914N13.3 (phosphodiesterase I/nucleotide pyrophosphatase 3) antibody E NPP 3 antibody E-NPP 3 antibody Ectonucleotide pyrophosphatase/phosphodiesterase 3 antibody Ectonucleotide pyrophosphatase/phosphodiesterase family member 3 antibody ENPP3 antibody ENPP3_HUMAN antibody gp130RB13 6 antibody gp130RB136 antibody M6S1 antibody NPP1 antibody NPP3 antibody NPPase antibody NPPS antibody Nucleotide pyrophosphatase antibody PC 1 antibody PC-1 antibody PCA1 antibody PD Ibeta antibody PD-Ibeta antibody PDNP1 antibody PDNP3 antibody Phosphodiesterase I beta antibody Phosphodiesterase I/nucleotide pyrophosphatase 3 antibody phosphodiesterase i/nucleotide pyrophosphatase beta antibody |
|---|---|
| Molecular Weight(MW) | 100.1kDa |
| Cellular Localization | Membrane. Secreted. Located to the apical surface in intestinal and kidney epithelial cells. Located to the cell surface of basophils, and to the apical plasma membrane of bile duct cells. Secreted in serum, and in lumen of epithelial cells. |
| SwissProt ID | O14638 |
|---|
Application
Western blot analysis of CD203C against human CD203C (AA: extra 45-163) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-93, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1710-93 against HEK293 (1) and CD203C (AA: extra 45-163)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-93, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Immunohistochemical analysis of paraffin-embedded human renal cancer tissue using anti-CD203C antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1710-93, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Flow cytometric analysis of CD203C was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-93, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | HL-60 cells renal cancer |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
