Application
Western blot analysis of CD48 against human CD48 (AA: 47-181) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-94, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of EM1710-94 against HEK293 (1) and CD48 (AA: 47-181)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-94, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.| Product Name | CD48 [3F5H10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Antigen CD48 antibody B cell membrane protein antibody B lymphocyte activation marker BLAST 1 antibody B-cell activation marker antibody B-lymphocyte activation marker BLAST-1 antibody BCM 1 surface antigen antibody BCM1 antibody BCM1 surface antigen antibody BLAST 1 antibody BLAST antibody BLAST1 antibody CD 48 antibody CD48 antibody CD48 antigen (B cell membrane protein) antibody CD48 antigen antibody CD48 molecule antibody CD48 protein antibody CD48_HUMAN antibody hCD48 antibody Leucocyte antigen MEM 102 antibody Leukocyte antigen MEM-102 antibody mCD48 antibody MEM 102 antibody MEM-102 antibody MEM102 antibody Signaling lymphocytic activation molecule 2 antibody SLAM family member 2 antibody SLAMF 2 antibody SLAMF2 antibody TCT.1 antibody |
| Product description | This gene encodes a member of the CD2 subfamily of immunoglobulin-like receptors which includes SLAM (signaling lymphocyte activation molecules) proteins. The encoded protein is found on the surface of lymphocytes and other immune cells, dendritic cells and endothelial cells, and participates in activation and differentiation pathways in these cells. The encoded protein does not have a transmembrane domain, however, but is held at the cell surface by a GPI anchor via a C-terminal domain which maybe cleaved to yield a soluble form of the receptor. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Immunogen | Purified recombinant fragment of human CD48 (AA: 47-181) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500-1:2,000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | Antigen CD48 antibody B cell membrane protein antibody B lymphocyte activation marker BLAST 1 antibody B-cell activation marker antibody B-lymphocyte activation marker BLAST-1 antibody BCM 1 surface antigen antibody BCM1 antibody BCM1 surface antigen antibody BLAST 1 antibody BLAST antibody BLAST1 antibody CD 48 antibody CD48 antibody CD48 antigen (B cell membrane protein) antibody CD48 antigen antibody CD48 molecule antibody CD48 protein antibody CD48_HUMAN antibody hCD48 antibody Leucocyte antigen MEM 102 antibody Leukocyte antigen MEM-102 antibody mCD48 antibody MEM 102 antibody MEM-102 antibody MEM102 antibody Signaling lymphocytic activation molecule 2 antibody SLAM family member 2 antibody SLAMF 2 antibody SLAMF2 antibody TCT.1 antibody |
|---|---|
| Molecular Weight(MW) | 27.7kDa |
| Cellular Localization | Cell membrane. |
| SwissProt ID | P09326 |
|---|
Application
Western blot analysis of CD48 against human CD48 (AA: 47-181) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-94, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1710-94 against HEK293 (1) and CD48 (AA: 47-181)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-94, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.| Positive Control | Highest expression level in leukocyte. |
|---|---|
| Application Notes | WB:1:500-1:2,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
