Application
Western blot analysis of CD335 against human CD335 (AA: extra 22-258) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-95, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of EM1710-95 against HEK293 (1) and CD335 (AA: extra 22-258)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-95, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of CD335 was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-95, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | CD335 [2B11E12] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Activating NK receptor NK p46 antibody CD335 antibody FLJ99094 antibody hNKp46 antibody Ly94 antibody Lymphocyte antigen 94 antibody Lymphocyte antigen 94 homolog (activating NK receptor; NK p46) antibody Lymphocyte antigen 94 homolog antibody Natural cytotoxicity triggering receptor 1 antibody Natural killer cell p46-related protein antibody NCR1 antibody NCT1 antibody NCTR1_HUMAN antibody NK cell activating receptor antibody NK cell-activating receptor antibody NK-p46 antibody NKp46 antibody |
| Product description | NCR1 (Natural Cytotoxicity Triggering Receptor 1) is a Protein Coding gene. Diseases associated with NCR1 include Viral Infectious Disease. Among its related pathways are Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell and Natural Killer Cell Receptors: Human Target Cell – NK Cell Ligand-Receptor Interactions. GO annotations related to this gene include signal transducer activity, downstream of receptor. An important paralog of this gene is LILRB3. |
| Immunogen | Purified recombinant fragment of human CD335 (AA: extra 22-258) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | Activating NK receptor NK p46 antibody
CD335 antibody
FLJ99094 antibody
hNKp46 antibody
Ly94 antibody
Lymphocyte antigen 94 antibody
Lymphocyte antigen 94 homolog (activating NK receptor NK p46) antibody Lymphocyte antigen 94 homolog antibody Natural cytotoxicity triggering receptor 1 antibody Natural killer cell p46-related protein antibody NCR1 antibody NCT1 antibody NCTR1_HUMAN antibody NK cell activating receptor antibody NK cell-activating receptor antibody NK-p46 antibody NKp46 antibody |
|---|---|
| Molecular Weight(MW) | 34.5kDa |
| Cellular Localization | Cell membrane. |
| SwissProt ID | O76036 |
|---|
Application
Western blot analysis of CD335 against human CD335 (AA: extra 22-258) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-95, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1710-95 against HEK293 (1) and CD335 (AA: extra 22-258)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-95, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of CD335 was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-95, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | HL-60 cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
