Application
Western blot analysis of CD66B against human CD66B (AA: 43-178) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-98, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of EM1710-98 against HEK293 (1) and CD66B (AA: 43-178)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-98, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.| Product Name | CD66B [2F12E8] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Carcinoembryonic antigen CGM6 antibody Carcinoembryonic antigen gene family member 6 antibody Carcinoembryonic antigen related cell adhesion molecule 8 antibody Carcinoembryonic antigen-related cell adhesion molecule 8 antibody CD 66b antibody CD 67 antibody CD66b antibody CD66b antigen antibody CD67 antibody CD67 antigen antibody CEACAM 8 antibody CEACAM8 antibody CEAM8_HUMAN antibody CGM 6 antibody CGM6 antibody NCA 95 antibody NCA95 antibody Non-specific cross-reacting antigen NCA-95 antibody Nonspecific cross reacting antigen NCA 95 antibody Nonspecific cross reacting antigen NCA95 antibody |
| Product description | CEACAM8 (Carcinoembryonic Antigen Related Cell Adhesion Molecule 8) is a Protein Coding gene. Diseases associated with CEACAM8 include Endocarditis and Shwartzman Phenomenon. Among its related pathways are Innate Immune System and Cell surface interactions at the vascular wall. An important paralog of this gene is CEACAM1. |
| Immunogen | Purified recombinant fragment of human CD66B (AA: 43-178) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500-1:2,000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | Carcinoembryonic antigen CGM6 antibody Carcinoembryonic antigen gene family member 6 antibody Carcinoembryonic antigen related cell adhesion molecule 8 antibody Carcinoembryonic antigen-related cell adhesion molecule 8 antibody CD 66b antibody CD 67 antibody CD66b antibody CD66b antigen antibody CD67 antibody CD67 antigen antibody CEACAM 8 antibody CEACAM8 antibody CEAM8_HUMAN antibody CGM 6 antibody CGM6 antibody NCA 95 antibody NCA95 antibody Non-specific cross-reacting antigen NCA-95 antibody Nonspecific cross reacting antigen NCA 95 antibody Nonspecific cross reacting antigen NCA95 antibody |
|---|---|
| Molecular Weight(MW) | 38.2kDa |
| Cellular Localization | Cell membrane. |
| SwissProt ID | P31997 |
|---|
Application
Western blot analysis of CD66B against human CD66B (AA: 43-178) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-98, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1710-98 against HEK293 (1) and CD66B (AA: 43-178)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-98, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.| Positive Control | Expressed in leukocytes of chronic myeloid Leukemia patients and bone marrow. |
|---|---|
| Application Notes | WB:1:500-1:2,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
