Application
Western blot analysis of CD64 against human CD64 (AA: extra 16-145) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-99, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of EM1710-99 against HEK293 (1) and CD64 (AA: extra 16-145)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-99, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-CD64 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1710-99, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Application
Flow cytometric analysis of CD64 was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-99, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | CD64 [5F5F8] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD 64 antibody CD64 antibody CD64 antigen antibody CD64A antibody CD64b antibody CD64c antibody Fc fragment of IgG high affinity Ia receptor antibody Fc fragment of IgG high affinity Ib receptor antibody Fc fragment of IgG high affinity Ic receptor antibody Fc fragment of IgG, high affinity Ia, receptor (CD64) antibody Fc fragment of IgG, high affinity Ia, receptor for (CD64) antibody Fc fragment of IgG, high affinity Ia, receptor for antibody Fc fragment of IgG, high affinity Ib, receptor (CD64) antibody Fc fragment of IgG, high affinity Ib, receptor for antibody Fc fragment of IgG, high affinity Ic, receptor (CD64) antibody Fc fragment of IgG, high affinity Ic, receptor (CD64), pseudogene antibody Fc fragment of IgG, high affinity Ic, receptor for antibody Fc gamma receptor antibody Fc gamma receptor I A1 antibody Fc gamma receptor I antibody Fc gamma receptor I B2 antibody Fc gamma RI antibody Fc gamma RIA antibody Fc gamma RIB antibody Fc of IgG high affinity Ia receptor antibody Fc-gamma RI antibody Fc-gamma RIA antibody Fc-gamma RIC antibody FCG 1 antibody FCG1 antibody FcgammaRI antibody FcgammaRIa antibody FCGR 1 antibody FCGR1 antibody FCGR1_HUMAN antibody FCGR1A antibody FCGR1B antibody FCGR1C antibody FcRI antibody FcRIB antibody FCRIC antibody FLJ18345 antibody HFcgammaRIB antibody High affinity immunoglobulin gamma Fc receptor I antibody High affinity immunoglobulin gamma Fc receptor IB antibody IGFR 1 antibody IGFR1 antibody IGFRB antibody IGFRC antibody IgG Fc receptor I antibody IgG Fc receptor IB antibody IgG Fc receptor IC antibody Immunoglobulin G Fc receptor I antibody Immunoglobulin G Fc receptor IB antibody Immunoglobulin G Fc receptor IC antibody MGC137713 antibody |
| Product description | This gene encodes a protein that plays an important role in the immune response. This protein is a high-affinity Fc-gamma receptor. The gene is one of three related gene family members located on chromosome 1. |
| Immunogen | Purified recombinant fragment of human CD64 (AA: extra 16-145) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | CD 64 antibody
CD64 antibody
CD64 antigen antibody
CD64A antibody
CD64b antibody
CD64c antibody
Fc fragment of IgG high affinity Ia receptor antibody
Fc fragment of IgG high affinity Ib receptor antibody
Fc fragment of IgG high affinity Ic receptor antibody
Fc fragment of IgG high affinity Ia receptor (CD64) antibody Fc fragment of IgG high affinity Ia receptor for (CD64) antibody Fc fragment of IgG high affinity Ia receptor for antibody Fc fragment of IgG high affinity Ib receptor (CD64) antibody Fc fragment of IgG high affinity Ib receptor for antibody Fc fragment of IgG high affinity Ic receptor (CD64) antibody Fc fragment of IgG high affinity Ic receptor (CD64) pseudogene antibody Fc fragment of IgG high affinity Ic receptor for antibody Fc gamma receptor antibody Fc gamma receptor I A1 antibody Fc gamma receptor I antibody Fc gamma receptor I B2 antibody Fc gamma RI antibody Fc gamma RIA antibody Fc gamma RIB antibody Fc of IgG high affinity Ia receptor antibody Fc-gamma RI antibody Fc-gamma RIA antibody Fc-gamma RIC antibody FCG 1 antibody FCG1 antibody FcgammaRI antibody FcgammaRIa antibody FCGR 1 antibody FCGR1 antibody FCGR1_HUMAN antibody FCGR1A antibody FCGR1B antibody FCGR1C antibody FcRI antibody FcRIB antibody FCRIC antibody FLJ18345 antibody HFcgammaRIB antibody High affinity immunoglobulin gamma Fc receptor I antibody High affinity immunoglobulin gamma Fc receptor IB antibody IGFR 1 antibody IGFR1 antibody IGFRB antibody IGFRC antibody IgG Fc receptor I antibody IgG Fc receptor IB antibody IgG Fc receptor IC antibody Immunoglobulin G Fc receptor I antibody Immunoglobulin G Fc receptor IB antibody Immunoglobulin G Fc receptor IC antibody MGC137713 antibody |
|---|---|
| Molecular Weight(MW) | 42.6kDa |
| Cellular Localization | Cell membrane. Stabilized at the cell membrane through interaction with FCER1G. |
| SwissProt ID | P12314 |
|---|
Application
Western blot analysis of CD64 against human CD64 (AA: extra 16-145) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-99, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1710-99 against HEK293 (1) and CD64 (AA: extra 16-145)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-99, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-CD64 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1710-99, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Flow cytometric analysis of CD64 was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-99, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | HL-60 cells bladder cancer |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
