Application
Western blot analysis of PTPRM against human PTPRM (AA: extra 565-742) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-04, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of EM1711-04 against HEK293 (1) and PTPRM (AA: extra 565-742)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-04, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.| Product Name | PTPRM [1H6D10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PTPRL 1 antibody hR PTPu antibody Protein tyrosine phosphatase mu antibody Protein tyrosine phosphatase mu precursor antibody Protein tyrosine phosphatase receptor type M antibody Protein tyrosine phosphatase receptor type mu polypeptide antibody Protein-tyrosine phosphatase mu antibody PTPRL1 antibody Ptprm antibody PTPRM protein antibody PTPRM_HUMAN antibody R PTP mu antibody R-PTP-mu antibody Receptor type tyrosine protein phosphatase mu antibody Receptor-type tyrosine-protein phosphatase mu antibody RPTP mu antibody RPTPM antibody RPTPU antibody |
| Product description | The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and two tandem catalytic domains, and thus represents a receptor-type PTP. The extracellular region contains a meprin-A5 antigen-PTP mu (MAM) domain, an Ig-like domain and four fibronectin type III-like repeats. This PTP has been shown to mediate cell-cell aggregation through the interaction with another molecule of this PTP on an adjacent cell. This PTP can interact with scaffolding protein RACK1/GNB2L1, which may be necessary for the downstream signaling in response to cell-cell adhesion. Alternative splicing results in multiple transcripts encoding distinct isoforms. |
| Immunogen | Purified recombinant fragment of human PTPRM (AA: extra 565-742) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500-1:2,000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | PTPRL 1 antibody hR PTPu antibody Protein tyrosine phosphatase mu antibody Protein tyrosine phosphatase mu precursor antibody Protein tyrosine phosphatase receptor type M antibody Protein tyrosine phosphatase receptor type mu polypeptide antibody Protein-tyrosine phosphatase mu antibody PTPRL1 antibody Ptprm antibody PTPRM protein antibody PTPRM_HUMAN antibody R PTP mu antibody R-PTP-mu antibody Receptor type tyrosine protein phosphatase mu antibody Receptor-type tyrosine-protein phosphatase mu antibody RPTP mu antibody RPTPM antibody RPTPU antibody |
|---|---|
| Molecular Weight(MW) | 163.7kDa |
| Cellular Localization | Membrane. |
| SwissProt ID | P28827 |
|---|
Application
Western blot analysis of PTPRM against human PTPRM (AA: extra 565-742) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-04, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1711-04 against HEK293 (1) and PTPRM (AA: extra 565-742)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-04, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.| Positive Control | Highest expression level in visceral pleura. |
|---|---|
| Application Notes | WB:1:500-1:2,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
