Application
Western blot analysis of CD16B against human CD16B (AA: 17-200) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-05, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of EM1711-05 against HEK293 (1) and CD16B (AA: 17-200)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-05, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of CD16B was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1711-05, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | CD16B [8F1C10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD16 antibody CD16b antibody CD16b antigen antibody Fc fragment of IgG low affinity IIIb receptor antibody Fc gamma receptor IIIb antibody Fc gamma RIII antibody Fc gamma RIII beta antibody Fc gamma RIIIb antibody Fc-gamma RIII antibody Fc-gamma RIII-beta antibody Fc-gamma RIIIb antibody FCG3 antibody FCG3B_HUMAN antibody FCGR3 antibody FCGR3B antibody FcR 10 antibody FcR-10 antibody FcR10 antibody FcRIII antibody FcRIIIb antibody IGFR3 antibody IgG Fc receptor III 1 antibody IgG Fc receptor III-1 antibody Low affinity immunoglobulin gamma Fc region receptor III B antibody Low affinity immunoglobulin gamma Fc region receptor III-B antibody |
| Product description | CD16, also known as FcγRIII, is a cluster of differentiation molecule found on the surface of natural killer cells, neutrophils, monocytes, and macrophages. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), which participate in signal transduction. The most well-researched membrane receptor implicated in triggering lysis by NK cells, CD16 is a molecule of the immunoglobulin superfamily (IgSF) involved in antibody-dependent cellular cytotoxicity (ADCC). It can be used to isolate populations of specific immune cells through fluorescent-activated cell sorting (FACS) or magnetic-activated cell sorting, using antibodies directed towards CD16. CD16 is the type III Fcγ receptor. In humans, it exists in two different forms: FcγRIIIa (CD16a) and FcγRIIIb (CD16b), which have 96% sequence similarity in the extracellular immunoglobulin binding regions. While FcγRIIIa is expressed on mast cells, macrophages, and natural killer cells as a transmembrane receptor, FcγRIIIb is only expressed on neutrophils. In addition, FcγRIIIb is the only Fc receptor anchored to the cell membrane by a glycosyl-phosphatidylinositol (GPI) linker, and also plays a significant role in triggering calcium mobilization and neutrophil degranulation. FcγRIIIa and FcγRIIIb together are able to activate degranulation, phagocytosis, and oxidative burst, which allows neutrophils to clear opsonized pathogens. |
| Immunogen | Purified recombinant fragment of human CD16B (AA: 17-200) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | CD16 antibody CD16b antibody CD16b antigen antibody Fc fragment of IgG low affinity IIIb receptor antibody Fc gamma receptor IIIb antibody Fc gamma RIII antibody Fc gamma RIII beta antibody Fc gamma RIIIb antibody Fc-gamma RIII antibody Fc-gamma RIII-beta antibody Fc-gamma RIIIb antibody FCG3 antibody FCG3B_HUMAN antibody FCGR3 antibody FCGR3B antibody FcR 10 antibody FcR-10 antibody FcR10 antibody FcRIII antibody FcRIIIb antibody IGFR3 antibody IgG Fc receptor III 1 antibody IgG Fc receptor III-1 antibody Low affinity immunoglobulin gamma Fc region receptor III B antibody Low affinity immunoglobulin gamma Fc region receptor III-B antibody |
|---|---|
| Molecular Weight(MW) | 26.2kDa |
| Cellular Localization | Cell membrane, Secreted |
| SwissProt ID | O75015 |
|---|
Application
Western blot analysis of CD16B against human CD16B (AA: 17-200) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-05, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1711-05 against HEK293 (1) and CD16B (AA: 17-200)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-05, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of CD16B was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1711-05, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | Hela cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
