Application
Western blot analysis of PTPRU against human PTPRU (AA: extra 579-749) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-06, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of EM1711-06 against HEK293 (1) and PTPRU (AA: extra 579-749)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-06, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.| Product Name | PTPRU [1A6E5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Pancreatic carcinoma phosphatase 2 antibody PCP-2 antibody Protein-tyrosine phosphatase J antibody PTP-J antibody PTPRU antibody R-PTP-U antibody Receptor-type tyrosine-protein phosphatase U antibody |
| Product description | The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and two tandem intracellular catalytic domains, and thus represents a receptor-type PTP. The extracellular region contains a meprin-A5 antigen-PTP (MAM) domain, Ig-like and fibronectin type III-like repeats. This PTP was thought to play roles in cell-cell recognition and adhesion. Studies of the similar gene in mice suggested the role of this PTP in early neural development. The expression of this gene was reported to be regulated by phorbol myristate acetate (PMA) or calcium ionophore in Jurkat T lymphoma cells. Alternatively spliced transcript variants have been reported. |
| Immunogen | Purified recombinant fragment of human PTPRU (AA: extra 579-749) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500-1:2,000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | Pancreatic carcinoma phosphatase 2 antibody PCP-2 antibody Protein-tyrosine phosphatase J antibody PTP-J antibody PTPRU antibody R-PTP-U antibody Receptor-type tyrosine-protein phosphatase U antibody |
|---|---|
| Molecular Weight(MW) | 162.4kDa |
| Cellular Localization | Type 1 membrane protein |
| SwissProt ID | q92729 |
|---|
Application
Western blot analysis of PTPRU against human PTPRU (AA: extra 579-749) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-06, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1711-06 against HEK293 (1) and PTPRU (AA: extra 579-749)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-06, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.| Positive Control | High levels in brain, pancreas, and skeletal muscle. |
|---|---|
| Application Notes | WB:1:500-1:2,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
