Application
Western blot analysis of TNFRSF11A against human TNFRSF11A (AA: extra 30-212) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-07, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of EM1711-07 against HEK293 (1) and TNFRSF11A (AA: extra 30-212)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-07, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of TNFRSF11A was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1711-07, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).Application
Flow cytometric analysis of TNFRSF11A was done on HL-60 cells . The cells were fixed, permeabilized and stained with the primary antibody (EM1711-07, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | TNFRSF11A [8H8B2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD 265 antibody CD265 antibody FEO antibody LOH18CR1 antibody Loss of heterozygosity 18 chromosomal region 1 antibody mRANK antibody ODFR antibody OFE antibody OPTB7 antibody Osteoclast differentiation factor receptor antibody OSTS antibody Paget disease of bone 2 antibody PDB 2 antibody PDB2 antibody RANK antibody Receptor activator of NF KB antibody Receptor activator of NF-KB antibody receptor activator of nuclear factor kappa B antibody TNF receptor superfamily member 11a antibody TNFRSF11A antibody TNR11_HUMAN antibody TRANCER antibody Tumor necrosis factor receptor superfamily member 11a NFKB activator antibody Tumor necrosis factor receptor superfamily member 11a activator of NFKB antibody Tumor necrosis factor receptor superfamily member 11A antibody |
| Product description | The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptors can interact with various TRAF family proteins, through which this receptor induces the activation of NF-kappa B and MAPK8/JNK. This receptor and its ligand are important regulators of the interaction between T cells and dendritic cells. This receptor is also an essential mediator for osteoclast and lymph node development. Mutations at this locus have been associated with familial expansile osteolysis, autosomal recessive osteopetrosis, and Paget disease of bone. Alternatively spliced transcript variants have been described for this locus. |
| Immunogen | Purified recombinant fragment of human TNFRSF11A (AA: extra 30-212) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | CD 265 antibody CD265 antibody FEO antibody LOH18CR1 antibody Loss of heterozygosity 18 chromosomal region 1 antibody mRANK antibody ODFR antibody OFE antibody OPTB7 antibody Osteoclast differentiation factor receptor antibody OSTS antibody Paget disease of bone 2 antibody PDB 2 antibody PDB2 antibody RANK antibody Receptor activator of NF KB antibody Receptor activator of NF-KB antibody receptor activator of nuclear factor kappa B antibody TNF receptor superfamily member 11a antibody TNFRSF11A antibody TNR11_HUMAN antibody TRANCER antibody Tumor necrosis factor receptor superfamily member 11a NFKB activator antibody Tumor necrosis factor receptor superfamily member 11a activator of NFKB antibody Tumor necrosis factor receptor superfamily member 11A antibody |
|---|---|
| Molecular Weight(MW) | 66kDa |
| Cellular Localization | Membrane. |
| SwissProt ID | Q9Y6Q6 |
|---|
Application
Western blot analysis of TNFRSF11A against human TNFRSF11A (AA: extra 30-212) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-07, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1711-07 against HEK293 (1) and TNFRSF11A (AA: extra 30-212)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-07, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of TNFRSF11A was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1711-07, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
Application
Flow cytometric analysis of TNFRSF11A was done on HL-60 cells . The cells were fixed, permeabilized and stained with the primary antibody (EM1711-07, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | Hela cells HL-60 cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
