Application
Western blot analysis of LILRA5 against human LILRA5 (AA: extra 42-268) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-08, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of EM1711-08 against HEK293 (1) and LILRA5 (AA: extra 42-268)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-08, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.| Product Name | LILRA5 [1B5D10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD85 antibody CD85 antigen-like family member F antibody CD85f antibody ILT-11 antibody ILT11 antibody Immunoglobulin like transcript 11 protein antibody Immunoglobulin-like transcript 11 antibody Leukocyte Ig like receptor 9 antibody Leukocyte immunoglobulin like receptor subfamily A (with TM domain) member 5 antibody Leukocyte immunoglobulin-like receptor 9 antibody Leukocyte immunoglobulin-like receptor subfamily A member 5 antibody Leukocyte immunoglobulin-like receptor subfamily B (with TM and ITIM domains) member 7 antibody LILRA5 antibody LILRB7 antibody LIR-9 antibody LIR9 antibody LIRA5_HUMAN antibody |
| Product description | The protein encoded by this gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family. LIR family members are known to have activating and inibitory functions in leukocytes. Crosslink of this receptor protein on the surface of monocytes has been shown to induce calcium flux and secretion of several proinflammatory cytokines, which suggests the roles of this protein in triggering innate immune responses. This gene is one of the leukocyte receptor genes that form a gene cluster on the chromosomal region 19q13.4. Four alternatively spliced transcript variants encoding distinct isoforms have been described. |
| Immunogen | Purified recombinant fragment of human LILRA5 (AA: extra 42-268) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500-1:2,000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | CD85 antibody CD85 antigen-like family member F antibody CD85f antibody ILT-11 antibody ILT11 antibody Immunoglobulin like transcript 11 protein antibody Immunoglobulin-like transcript 11 antibody Leukocyte Ig like receptor 9 antibody Leukocyte immunoglobulin like receptor subfamily A (with TM domain) member 5 antibody Leukocyte immunoglobulin-like receptor 9 antibody Leukocyte immunoglobulin-like receptor subfamily A member 5 antibody Leukocyte immunoglobulin-like receptor subfamily B (with TM and ITIM domains) member 7 antibody LILRA5 antibody LILRB7 antibody LIR-9 antibody LIR9 antibody LIRA5_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 32.8kDa |
| Cellular Localization | Secreted and Membrane. Secreted. |
| SwissProt ID | A6NI73 |
|---|
Application
Western blot analysis of LILRA5 against human LILRA5 (AA: extra 42-268) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-08, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1711-08 against HEK293 (1) and LILRA5 (AA: extra 42-268)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-08, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.| Positive Control | Expressed mostly in tissues of the hematopoietic system, including bone marrow, spleen, lymph node and peripheral leukocytes. |
|---|---|
| Application Notes | WB:1:500-1:2,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
