Application
Western blot analysis of LILRB2 against human LILRB2 (AA: 51-184) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-09, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of EM1711-09 against HEK293 (1) and LILRB2 (AA: 51-184)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-09, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of EM1711-09 against MOLT4 (1) and K562 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-09, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of LILRB2 was done on HL-60 cells . The cells were fixed, permeabilized and stained with the primary antibody (EM1711-09, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | LILRB2 [2G7E11] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD85 antigen-like family member D antibody CD85d antibody CD85d antigen antibody ILT 4 antibody ILT-4 antibody ILT4 antibody Immunoglobulin like transcript 4 antibody Immunoglobulin-like transcript 4 antibody Leukocyte immunoglobulin like receptor 2 antibody Leukocyte immunoglobulin like receptor subfamily B member 2 antibody Leukocyte immunoglobulin-like receptor 2 antibody Leukocyte immunoglobulin-like receptor subfamily B member 2 antibody Leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 2 antibody LILRA6 antibody LILRB2 antibody LIR 2 antibody LIR-2 antibody LIR2 antibody LIRb antibody LIRB2_HUMAN antibody MIR 10 antibody MIR-10 antibody MIR10 antibody Monocyte/macrophage immunoglobulin like receptor 10 antibody Monocyte/macrophage immunoglobulin-like receptor 10 antibody |
| Product description | This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Immunogen | Purified recombinant fragment of human LILRB2 (AA: 51-184) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | CD85 antigen-like family member D antibody
CD85d antibody
CD85d antigen antibody
ILT 4 antibody
ILT-4 antibody
ILT4 antibody
Immunoglobulin like transcript 4 antibody
Immunoglobulin-like transcript 4 antibody
Leukocyte immunoglobulin like receptor 2 antibody
Leukocyte immunoglobulin like receptor subfamily B member 2 antibody
Leukocyte immunoglobulin-like receptor 2 antibody
Leukocyte immunoglobulin-like receptor subfamily B member 2 antibody
Leukocyte immunoglobulin-like receptor subfamily B (with TM and ITIM domains) member 2 antibody LILRA6 antibody LILRB2 antibody LIR 2 antibody LIR-2 antibody LIR2 antibody LIRb antibody LIRB2_HUMAN antibody MIR 10 antibody MIR-10 antibody MIR10 antibody Monocyte/macrophage immunoglobulin like receptor 10 antibody Monocyte/macrophage immunoglobulin-like receptor 10 antibody |
|---|---|
| Molecular Weight(MW) | 65kDa |
| Cellular Localization | Membrane. |
| SwissProt ID | Q8N423 |
|---|
Application
Western blot analysis of LILRB2 against human LILRB2 (AA: 51-184) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-09, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1711-09 against HEK293 (1) and LILRB2 (AA: 51-184)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-09, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1711-09 against MOLT4 (1) and K562 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-09, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of LILRB2 was done on HL-60 cells . The cells were fixed, permeabilized and stained with the primary antibody (EM1711-09, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | MOLT4 K562 cell HL-60 cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
