Application
Western blot analysis of CCL4 against human CCL4 (AA: 24-92) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-11, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of EM1711-11 against HEK293 (1) and CCL4 (AA: 24-92)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-11, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of CCL4 was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1711-11, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | CCL4 [7C9E4] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MIP 1 beta antibody Secreted protein G 26 antibody ACT 2 antibody ACT-2 antibody ACT2 antibody AT744.1 antibody AT744.2 antibody C C motif chemokine 4 antibody C C motif chemokine 4 like antibody C C motif chemokine ligand 4 like 1 antibody C C motif chemokine ligand 4 like 2 antibody CC chemokine ligand 4 antibody CC chemokine ligand 4L1 antibody CC chemokine ligand 4L1d2 antibody CC chemokine ligand 4L2 antibody CCL4 antibody CCL4_HUMAN antibody ccl4l 1 antibody CCL4L antibody CCL4L1 antibody Chemokine (C C motif) ligand 4 antibody Chemokine (C C motif) ligand 4 like 1 antibody Chemokine (C C motif) ligand 4 like 1, telomeric antibody Chemokine (C C motif) ligand 4 like 2 antibody Chemokine CC Motif Ligand 4 antibody G 26 antibody G 26 T lymphocyte secreted protein antibody G-26 T-lymphocyte-secreted protein antibody HC21 antibody Immune activation 2 antibody LAG 1 antibody LAG-1 antibody LAG1 antibody Lymphocyte activation gene 1 antibody Lymphocyte activation gene 1 protein antibody Macrophage inflammatory protein 1 beta antibody Macrophage inflammatory protein 1-beta antibody Macrophage inflammatory protein 1b2 antibody MGC104418 antibody MGC126025 antibody MGC126026 antibody MIP-1-beta antibody MIP-1-beta(1-69) antibody MIP-1-beta(3-69) antibody MIP1 beta antibody MIP1B antibody MIP1B1 antibody Monocyte adherence induced protein 5 alpha antibody PAT 744 antibody Protein H400 antibody SCYA2 antibody SCYA4 antibody SCYA4L antibody SCYA4L1 antibody SCYA4L2 antibody SCYQ4L2 antibody Secreted protein G 26 antibody Secreted protein G26 antibody SIS gamma antibody SIS-gamma antibody Small inducible cytokine A4 (homologous to mouse Mip 1b) antibody Small inducible cytokine A4 antibody small inducible cytokine A4-like antibody Small-inducible cytokine A4 antibody T cell activation protein 2 antibody T-cell activation protein 2 antibody |
| Product description | Chemokine (C-C motif) ligands 4, also known as CCL4, is a protein which in humans is encoded by the CCL4 gene. CCL4, also known as Macrophage inflammatory protein-1β (MIP-1β) is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 is a major HIV-suppressive factor produced by CD8+ T cells. Perforin-low memory CD8+ T cells that normally synthesize MIP-1-beta. CCL4 is produced by: neutrophils, monocytes, B cells, T cells, fibroblasts, endothelial cells, and epithelial cells. Concentration of this chemokine has been shown to be inversely related with MicroRNA-125b. Concentration of CCL4 within the body increases with age, which may cause chronic inflammation and liver damage. The protein encoded by this gene is a mitogen-inducible monokine and is one of the major HIV-suppressive factors produced by CD8+ T-cells. The encoded protein is secreted and has chemokinetic and inflammatory functions. |
| Immunogen | Purified recombinant fragment of human CCL4 (AA: 24-92) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | MIP 1 beta antibody
Secreted protein G 26 antibody
ACT 2 antibody
ACT-2 antibody
ACT2 antibody
AT744.1 antibody
AT744.2 antibody
C C motif chemokine 4 antibody
C C motif chemokine 4 like antibody
C C motif chemokine ligand 4 like 1 antibody
C C motif chemokine ligand 4 like 2 antibody
CC chemokine ligand 4 antibody
CC chemokine ligand 4L1 antibody
CC chemokine ligand 4L1d2 antibody
CC chemokine ligand 4L2 antibody
CCL4 antibody
CCL4_HUMAN antibody
ccl4l 1 antibody
CCL4L antibody
CCL4L1 antibody
Chemokine (C C motif) ligand 4 antibody
Chemokine (C C motif) ligand 4 like 1 antibody
Chemokine (C C motif) ligand 4 like 1 telomeric antibody Chemokine (C C motif) ligand 4 like 2 antibody Chemokine CC Motif Ligand 4 antibody G 26 antibody G 26 T lymphocyte secreted protein antibody G-26 T-lymphocyte-secreted protein antibody HC21 antibody Immune activation 2 antibody LAG 1 antibody LAG-1 antibody LAG1 antibody Lymphocyte activation gene 1 antibody Lymphocyte activation gene 1 protein antibody Macrophage inflammatory protein 1 beta antibody Macrophage inflammatory protein 1-beta antibody Macrophage inflammatory protein 1b2 antibody MGC104418 antibody MGC126025 antibody MGC126026 antibody MIP-1-beta antibody MIP-1-beta(1-69) antibody MIP-1-beta(3-69) antibody MIP1 beta antibody MIP1B antibody MIP1B1 antibody Monocyte adherence induced protein 5 alpha antibody PAT 744 antibody Protein H400 antibody SCYA2 antibody SCYA4 antibody SCYA4L antibody SCYA4L1 antibody SCYA4L2 antibody SCYQ4L2 antibody Secreted protein G 26 antibody Secreted protein G26 antibody SIS gamma antibody SIS-gamma antibody Small inducible cytokine A4 (homologous to mouse Mip 1b) antibody Small inducible cytokine A4 antibody small inducible cytokine A4-like antibody Small-inducible cytokine A4 antibody T cell activation protein 2 antibody T-cell activation protein 2 antibody |
|---|---|
| Molecular Weight(MW) | 10.2kDa |
| Cellular Localization | Secreted. |
| SwissProt ID | P13236 |
|---|
Application
Western blot analysis of CCL4 against human CCL4 (AA: 24-92) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-11, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1711-11 against HEK293 (1) and CCL4 (AA: 24-92)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-11, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of CCL4 was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1711-11, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | HL-60 cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
