Application
Western blot analysis of CD109 against human CD109 (AA: extra 1274-1421) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-15, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of EM1711-15 against HEK293 (1) and CD109 (AA: extra 1274-1421)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-15, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of CD109 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1711-15, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | CD109 [5E11A12] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 150 KDa TGF beta 1 binding protein antibody 150 KDa TGF-beta-1-binding protein antibody 7D1 antibody 8A3 antibody Activated T cell marker CD109 antibody C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7 antibody CD 109 antibody CD109 antibody CD109 antigen antibody CD109_HUMAN antibody CPAMD7 antibody DKFZp762L1111 antibody E123 antibody FLJ38569 antibody FLJ41966 antibody Gov system alloantigens on platelets antibody p180 antibody Platelet specific Gov antigen antibody Platelet-specific Gov antigen antibody r150 antibody RP11 525G3.1 antibody |
| Product description | This gene encodes a glycosyl phosphatidylinositol (GPI)-linked glycoprotein that localizes to the surface of platelets, activated T-cells, and endothelial cells. CD109 is a GPI-linked cell surface antigen expressed by CD34+ acute myeloid leukemia cell lines, T-cell lines, activated T lymphoblasts, endothelial cells, and activated platelets (Lin et al., 2002). In addition, the platelet-specific Gov antigen system (HPA15),[6] implicated in refractoriness to platelet transfusion, neonatal alloimmune thrombocytopenia, and posttransfusion purpura, is carried by CD109 The protein binds to and negatively regulates signalling by transforming growth factor beta (TGF-beta). Multiple transcript variants encoding different isoforms have been found for this gene. |
| Immunogen | Purified recombinant fragment of human CD109 (AA: extra 1274-1421) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | 150 KDa TGF beta 1 binding protein antibody 150 KDa TGF-beta-1-binding protein antibody 7D1 antibody 8A3 antibody Activated T cell marker CD109 antibody C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7 antibody CD 109 antibody CD109 antibody CD109 antigen antibody CD109_HUMAN antibody CPAMD7 antibody DKFZp762L1111 antibody E123 antibody FLJ38569 antibody FLJ41966 antibody Gov system alloantigens on platelets antibody p180 antibody Platelet specific Gov antigen antibody Platelet-specific Gov antigen antibody r150 antibody RP11 525G3.1 antibody |
|---|---|
| Molecular Weight(MW) | 161.7kDa |
| Cellular Localization | Cell membrane. |
| SwissProt ID | Q6YHK3 |
|---|
Application
Western blot analysis of CD109 against human CD109 (AA: extra 1274-1421) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-15, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1711-15 against HEK293 (1) and CD109 (AA: extra 1274-1421)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-15, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of CD109 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1711-15, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | Jurkat cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
